EasySep™ Mouse CD45 Positive Selection Kit

Immunomagnetic positive selection kit

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EasySep™ Mouse CD45 Positive Selection Kit

Immunomagnetic positive selection of CD45+ leukocytes from mouse lung, splenocytes, or other tissues

2 x 109 cells
Catalog #18945
704 USD

Required Products

Overview

The EasySep™ Mouse CD45 Positive Selection Kit is designed to isolate CD45 cells from single-cell suspensions of splenocytes, lung, or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD45 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Advantages:
• Fast and easy-to-use
• Up to 98% purity
• No columns required
• Isolated cells are not fluorochrome-labeled
Components:
  • EasySep™ Mouse CD45 Positive Selection Kit (Catalog #18945)
    • EasySep™ Mouse CD45 Positive Selection Kit Component A, 1 mL
    • EasySep™ Mouse CD4 Positive Selection Kit Component B, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells; Dendritic Cells; Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells; Innate Lymphoid Cells; Leukemia/Lymphoma Cells; Lymphocytes; Macrophages; Megakaryocytes; Monocytes; Mononuclear Cells; Myeloid Cells; NK Cells; Plasma Cells; T Cells; T Cells, Other Subsets
Species:
Mouse
Sample Source:
Lung; Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(10)

Data and Publications

Data

FACS Profile Results with EasySep™ Mouse CD45 Positive Selection Kit FACS Profile Results with EasySep™ Mouse CD45 Positive Selection Kit

Figure 1. Typical EasySep™ Mouse CD45 Positive Selection Profile

(A) Starting with a naïve mouse lung single-cell suspension, the leukocyte content (CD45+) of the isolated fraction is typically 97.0% ± 1.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions in lung are 53.1% and 94.1%, respectively. (B) Starting with unlysed naïve mouse splenocytes, the leukocyte content (CD45+) of the isolated fraction is typically 97.6 ± 1.3% (mean ± SD using the purple EasySep™ Magnet). In the above example using spleen, the purities of the start and final isolated fractions are 31.2% and 96.6%, respectively.

STEMCELL TECHNOLOGIES INC.'S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.