Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21
Mouse monoclonal IgG1, kappa isotype control antibody
Request Pricing
Thank you for your interest in this product. Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
Overview
This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Data Figures
Figure 1. Data for Unconjugated
(A) Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, Catalog #60107) followed by a rat anti-mouse IgG1 antibody, PE.(B) Human embryonic stem (ES) cells were cultured using mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, followed by goat anti-mouse IgG, FITC (inset) or with a positive control antibody of the same isotype, Anti-Human SSEA-5 Antibody, Clone 8e11 (Catalog #60063), followed by goat anti-mouse IgG, FITC (green).
Figure 2. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, Alexa Fluor® 488, Catalog #60107AD). (B) Human induced pluripotent stem (iPS) cells were cultured using mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (inset) or with a positive control antibody of the same isotype, Anti-Human SSEA-5 Antibody, Clone 8e11, Alexa Fluor® 488 (Catalog #60063AD, green).
Figure 3. Data for APC-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, APC, Catalog #60107AZ).
Figure 4. Data for Biotin-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, Biotin, Catalog #60107BT) followed by SAV APC.
Figure 5. Data for FITC-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, FITC, Catalog #60107FI).
Figure 6. Data for PE-Conjugated
(A) Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, PE, Catalog #60107PE). (B) Human induced pluripotent stem (iPS) cells were cultured using mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (inset) or with a positive control antibody of the same isotype, Anti-Human SSEA-5 Antibody, Clone 8e11, PE (Catalog #60063PE, red).
Figure 7. Data for PS-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC- 21, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD45 Antibody, Clone HI30, PerCP-Cy5.5; Catalog #60018PS).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Publications (1)
Abstract
Related Products
Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
