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PneumaCult™ Apical-Out Airway Organoid Secretory Supplement

Supplement for the development of secretory cells in apical-out airway organoids

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PneumaCult™ Apical-Out Airway Organoid Secretory Supplement

Supplement for the development of secretory cells in apical-out airway organoids

From: 125 USD
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Supplement for the development of secretory cells in apical-out airway organoids
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Product Advantages


  • Generate apical-out organoids with basal, ciliated, and secretory cells for a more physiologically relevant airway model

  • Simplify your workflow with a Matrigel®-free protocol that enables easy organoid recovery and downstream analysis

  • Use in high-throughput applications such as drug screening and toxicity studies

  • Maintain consistency across experiments and donors with a serum- and BPE-free formulation for reliable performance

What's Included

  • PneumaCult™ Apical-Out Airway Organoid Secretory Supplement, 3 x 1 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. PneumaCult™ Apical-Out Airway Organoid Secretory Medium
    PneumaCult™ Apical-Out Airway Organoid Secr...

    Serum- and BPE-free medium for differentiation of human primary airway epithelial cells to mature apical-out airway organoids containing basal, ciliated, and secretory cells

Overview

Generate mature apical-out airway organoids containing basal, ciliated, and secretory cells in just 15 days using a simple, scalable, and Matrigel®-free protocol. As part of the PneumaCult™ Apical-Out Airway Organoid Secretory Medium (Catalog #100-2078) kit , this supplement is designed for use with human primary airway epithelial cells (HAECs) to promote the development of secretory cells in polarized airway organoids with apical-out architecture—providing a more physiologically relevant model for your pulmonary research.

Organoids can be generated in AggreWell™400 24-well plates (Catalog #34415), without the need for cell culture inserts. The formulation is serum- and BPE-free to support experimental consistency. For optimal results, begin your workflow by expanding HAECs in PneumaCult™-NGEx Medium (Catalog #100-1505) prior to differentiation.

Find more about the applications of lung organoids in our learning center.
Brand
PneumaCult

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
PneumaCult™ Apical-Out Airway Organoid Secretory Supplement
Catalog #
100-2181
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
PneumaCult™ Apical-Out Airway Organoid Secretory Supplement
Catalog #
100-2181
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids
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Resources and Publications

Educational Materials (7)

Pulmonary Research
Brochure
Pulmonary Research
Cellular Organization and Biology of the Respiratory System
Wallchart
Cellular Organization and Biology of the Respiratory System
Beating Cilia of an Apical-Out Airway Organoid Grown in PneumaCult™
0:20
Video
Beating Cilia of an Apical-Out Airway Organoid Grown in PneumaCult™
Organoid-Based Assays for Disease Modeling
24:55
Webinar
Organoid-Based Assays for Disease Modeling
Thinking Outside the Dome: Versatile Organotypic Cell Culture Methods
39:57
Webinar
Thinking Outside the Dome: Versatile Organotypic Cell Culture Methods
Human Airway Organoids to Study Host-Pathogen Interactions
35:24
Webinar
Human Airway Organoids to Study Host-Pathogen Interactions
Apical-Out Airway Organoids and Airway-Immune Co-Cultures Are Suitable Models for Antiviral Compound Screenings and to Study Infectious Diseases
Scientific Poster
Apical-Out Airway Organoids and Airway-Immune Co-Cultures Are Suitable Models for Antiviral Compound...

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