mTeSR™3D is a specialised and serum-free formulation. This culture system is compatible with a range of suspension culture vessels.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Figure 1. hPSC growth in mTeSR™3D
mTeSR™3D supports expansion of hES and hiPS cells over multiple passages in aggregate suspension culture. Shown are cumulative viable cell numbers for (A) hESC line H9 cultured in shaker bottles (10 passages) and (B) hiPSC line STiPS-F016 cultured in spinner flasks (5 passages). Some cell lines may experience 1 passage of adaptation from 2D to 3D, as shown in (B).
Figure 2. hPSC aggregate morphology in mTeSR3D
Representative images of hPSC aggregates cultured in mTeSR™3D. (A) H9 cells (B) H7 cells (C) STiPS-F016 cells.
Figure 3. Expression of undifferentiated cell markers
hPSCs cultured in mTeSR™3D maintain high expression of OCT4. (A) H9 cells after 10 passages in shaker bottles. (B) STiPS-F016 cells after 5 passages in spinner flasks. Representative flow cytometry plots shown.
Figure 4. Tri-lineage Differentiation
hPSCs cultured in mTeSR™3D can be efficiently differentiated to all three germ layers. Trilineage differentiation potential was assessed using the STEMdiff™ Trilineage Differentiation Kit (Cat # 05230).