mTeSR™3D

Serum-free media for suspension culture of human ES and iPS cells

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

mTeSR™3D

Serum-free media for suspension culture of human ES and iPS cells

mTeSR™3D
1 Kit
602 USD
Catalog # 03950

Serum-free media for suspension culture of human ES and iPS cells

What's Included

  • mTeSR™3D Seed Basal Medium, 400 mL
  • mTeSR™3D Seed 5X Supplement, 100 mL
  • mTeSR™3D Feed Supplement A, 100 mL
  • mTeSR™3D Feed Supplement B, 12 mL
  • New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
    Products for Your Protocol
    To see all required products for your protocol, please consult the Protocols and Documentation.

    Overview

    mTeSR™3D was developed for expansion and scale-up of undifferentiated human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells as aggregates in 3D suspension culture. The novel fed-batch workflow saves time and media. Daily feeds replenish nutrients and eliminate the need for medium exchanges on non-passaging days.

    mTeSR™3D is a specialised and serum-free formulation. This culture system is compatible with a range of suspension culture vessels.
    Subtype
    Specialized Media
    Cell Type
    Pluripotent Stem Cells
    Species
    Human
    Application
    Cell Culture, Expansion
    Brand
    TeSR
    Area of Interest
    Stem Cell Biology
    Formulation
    Serum-Free

    Data Figures

    Figure 1. hPSC growth in mTeSR™3D

    mTeSR™3D supports expansion of hES and hiPS cells over multiple passages in aggregate suspension culture. Shown are cumulative viable cell numbers for (A) hESC line H9 cultured in shaker bottles (10 passages) and (B) hiPSC line STiPS-F016 cultured in spinner flasks (5 passages). Some cell lines may experience 1 passage of adaptation from 2D to 3D, as shown in (B).

    Figure 2. hPSC aggregate morphology in mTeSR3D

    Representative images of hPSC aggregates cultured in mTeSR™3D. (A) H9 cells (B) H7 cells (C) STiPS-F016 cells.


    Figure 3. Expression of undifferentiated cell markers

    hPSCs cultured in mTeSR™3D maintain high expression of OCT4. (A) H9 cells after 10 passages in shaker bottles. (B) STiPS-F016 cells after 5 passages in spinner flasks. Representative flow cytometry plots shown.

    Figure 4. Tri-lineage Differentiation

    hPSCs cultured in mTeSR™3D can be efficiently differentiated to all three germ layers. Trilineage differentiation potential was assessed using the STEMdiff™ Trilineage Differentiation Kit (Cat # 05230).

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Product Name
    mTeSR™3D
    Catalog #
    03950
    Lot #
    All
    Language
    English
    Document Type
    Technical Manual
    Product Name
    mTeSR™3D
    Catalog #
    03950
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 1
    Product Name
    mTeSR™3D
    Catalog #
    03950
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 2
    Product Name
    mTeSR™3D
    Catalog #
    03950
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 3
    Product Name
    mTeSR™3D
    Catalog #
    03950
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 4
    Product Name
    mTeSR™3D
    Catalog #
    03950
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Contact STEMCELL Technologies

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