EasySep™ RBC Depletion Reagent

Immunomagnetic negative selection
Catalog #
18170_C
Immunomagnetic negative selection
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Overview

The EasySep™ RBC Depletion Reagent is designed to immunomagnetically deplete red blood cells (RBCs) from fresh human peripheral blood, umbilical cord blood, bone marrow or leukapheresis samples by negative selection. RBCs are targeted with Tetrameric Antibody Complexes recognizing Glycophorin A and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off.

This product replaces the EasySep™ Human Glycophorin A Depletion Kit (Catalog #18352) for even faster cell isolations.
Advantages
The benefits of this kit include:
• 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation or lysis
• Fast, easy-to-use and column-free
• Isolated cells are untouched
Components
  • EasySep™ RBC Depletion Reagent (Catalog #18170)
    • EasySep™ RBC Depletion Reagent, 10 mL
  • EasySep™ RBC Depletion Reagent for RoboSep™ (Catalog #18170RF)
    • EasySep™ RBC Depletion Reagent for RoboSep™, 4 x 2.5 mL
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Species
Human
Sample Source
Whole Blood
Selection Method
Negative
Brand
EasySep, RoboSep
Area of Interest
Chimerism, Drug Discovery and Toxicity Testing, HLA, Immunology

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ RBC Depletion Reagent - 10mL
Catalog #
18170
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ RBC Depletion Reagent - 10mL
Catalog #
18170
Lot #
All
Language
English
Document Type
Product Information Sheet 3
Product Name
EasySep™ RBC Depletion Reagent - 10mL
Catalog #
18170
Lot #
All
Language
English
Document Type
Product Information Sheet 4
Product Name
EasySep™ RBC Depletion Reagent - 10mL
Catalog #
18170
Lot #
All
Language
English
Document Type
Product Information Sheet 5
Product Name
EasySep™ RBC Depletion Reagent - 10mL
Catalog #
18170
Lot #
All
Language
English
Document Type
Product Information Sheet 1
Product Name
EasySep™ RBC Depletion Reagent for RoboSep
Catalog #
18170RF
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ RBC Depletion Reagent for RoboSep
Catalog #
18170RF
Lot #
All
Language
English
Document Type
Product Information Sheet 3
Product Name
EasySep™ RBC Depletion Reagent for RoboSep
Catalog #
18170RF
Lot #
All
Language
English
Document Type
Product Information Sheet 4
Product Name
EasySep™ RBC Depletion Reagent for RoboSep
Catalog #
18170RF
Lot #
All
Language
English
Document Type
Product Information Sheet 5
Product Name
EasySep™ RBC Depletion Reagent for RoboSep
Catalog #
18170RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ RBC Depletion Reagent - 10mL
Catalog #
18170
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ RBC Depletion Reagent - 10mL
Catalog #
18170
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ RBC Depletion Reagent for RoboSep
Catalog #
18170RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ RBC Depletion Reagent for RoboSep
Catalog #
18170RF
Lot #
All
Language
English

Educational Materials(14)

Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Brochure
Cell Enrichment Prior to Cell Sorting
Brochure
EasySep™ RBC Depletion Reagent
Brochure
Isolate Human Immune Cells
Wallchart
Production of Chimeric Antigen Receptor T Cells
Wallchart
Human Immune Cytokines
Wallchart
Antigen Processing and Presentation
Wallchart
The Immune Response to HIV Poster
Video
2:34
How to Isolate Cells in 96-Well Plates Using the EasyPlate™ Cell Separation Magnet
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Scientific Poster
Centrifugation and RBC Lysis-Free Preparation of Blood Samples in Under 30 Minutes
Load More Educational Materials

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. EasySep™ RBC Depletion Reagent Provides Superior RBC Depletion Compared to Ammonium Chloride Lysis

Different types of RBC-containing samples from normal healthy donors were processed to remove RBCs by using either ammonium chloride (NH4Cl) lysis or immunomagnetic depletion with EasySep™ RBC Depletion Reagent. After RBC removal, samples were stained with fluorochrome-conjugated anti-CD45 and anti-Glycophorin A antibodies and analyzed by flow cytometry. Residual RBCs were identified as Glycophorin A+/CD45 events. (A) The percentages of residual RBCs in various samples following use of EasySep™ RBC Depletion Reagent was significantly lower compared to samples treated with ammonium chloride (mean ± SD; n = 31). (B) Both, RBC lysis with ammonium chloride and RBC removal using EasySep™ RBC Depletion Reagent resulted in an equivalent total number of white blood cells recovered from whole blood samples (mean ± SD; n = 37).

Figure 2. Typical RBC Removal Using EasySep™ RBC Depletion Reagent With Human Whole Blood Samples

Starting with human whole blood from normal healthy donors, the percentage of residual RBCs (Glycophorin A+/CD45-) following use of EasySep™ RBC Depletion Reagent is typically 2 ± 3 (mean ± SD; n = 31). In the above example, the residual RBC content is 0.05%.

Figure 3. Typical RBC Removal Using EasySep™ RBC Depletion Reagent With Human Bone Marrow Samples

Starting with human bone marrow, the percentage of residual RBCs (Glycophorin A+/CD45-) following use of EasySep™ RBC Depletion Reagent is typically 0.39 ± 0.34 (mean ± SD; n = 5). In the above example, the residual RBC content is 0.57%.

Figure 4. Typical RBC Removal Using EasySep™ RBC Depletion Reagent With Buffy Coat Samples

Starting with buffy coat, the percentage of residual RBCs (Glycophorin A+/CD45-) following use of EasySep™ RBC Depletion Reagent is typically 0.12 ± 0.08 (mean ± SD; n = 6). In the above example, the residual RBC content is 0.26%.

Figure 5. Typical RBC Removal Using EasySep™ RBC Depletion Reagent With Cord Blood Samples

Starting with cord blood, the percentage of residual RBCs (Glycophorin A+/CD45-) following use of EasySep™ RBC Depletion Reagent is typically 1.7 ± 2.2 (mean ± SD; n = 16). In the above example, the residual RBC content is 0.08%.

Figure 6. Typical RBC Removal Using EasySep™ RBC Depletion Reagent With Leukapheresis Samples

Starting with leukapheresis samples, the percentage of residual RBCs (Glycophorin A+/CD45-) following use of EasySep™ RBC Depletion Reagent is typically 0.5 ± 0.8 (mean ± SD; n = 15). In the above example, the residual RBC content is 0.6%.

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