Anti-Mouse CD44 Antibody, Clone IM7

Rat monoclonal IgG2b antibody against human, mouse, rhesus CD44 (tissue non-specific alkaline phosphatase)
From: 65 CAD

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Rat monoclonal IgG2b antibody against human, mouse, rhesus CD44 (tissue non-specific alkaline phosphatase)
From: 65 CAD

Overview

The IM7 antibody reacts with CD44 (Ly-24), an ~80 - 95 kDa type 1 transmembrane glycoprotein involved in cell-cell and cell-matrix interactions. CD44 is expressed on the surface of many cells, including leukocytes, hepatocytes, endothelial, epithelial, and mesenchymal cells. Expression levels increase upon activation of T and B cells, and memory cells exhibit a CD44[High] phenotype. CD44 binds many ligands, including hyaluronic acid, collagen, fibronectin, growth factors and metalloproteinases, thus modulating processes such as lymphocyte activation, recirculation and homing, leukocyte rolling and aggregation, hematopoiesis, and tumor metastasis. Numerous disorders are associated with altered expression or dysfunction of CD44. Many CD44 isoforms have been identified, with alternative splicing, differential N- and O- glycosylation, and sulfation mediating the functional role(s) played by the protein in a specific cell. The IM7 monoclonal antibody reacts with an extracellular epitope found on all isoforms of CD44 and both murine allotypes.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD4+ T Cell Enrichment Kit (Catalog #19752).
Subtype:
Primary Antibodies
Target Antigen:
CD44
Reactive Species:
Baboon; Cat; Chimpanzee; Cow; Cynomolgus; Dog; Horse; Human; Mouse; Pig; Rhesus; Squirrel Monkey
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated
Host Species:
Rat
Cell Type:
Mammary Cells; T Cells
Application:
Cell Isolation; CyTOF; ELISA; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation
Area of Interest:
Epithelial Cell Biology; Immunology; Stem Cell Biology
Clone:
IM7
Gene ID:
12505/960
Isotype:
IgG2b, kappa

Technical Resources

Educational Materials

(3)

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Mouse CD44 Antibody, Clone IM7, followed by goat antimouse IgG, FITC (filled histogram) or a rat IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC (open histogram). Data was gated on the total lymphocyte population.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (open histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Mouse CD44 Antibody, Clone IM7, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (open histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, Biotin followed by streptavidin (SAV) APC (filled histogram) or a rat IgG2b, kappa Biotin isotype control antibody followed by SAV APC (solid line histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Mouse CD44 Antibody, Clone IM7, Biotin followed by streptavidin (SAV) APC (filled histogram) or a rat IgG2b, kappa Biotin isotype control antibodyfollowed by SAV APC (open histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (open histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Mouse CD44 Antibody, Clone IM7, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (open histogram).

Data for PerCP-Cy55-Conjugated

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, FITC (filled histogram) or a rat IgG2b, kappa isotype control antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 7. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, APC (filled histogram) or a rat IgG2b, kappa isotype control antibody, APC (solid line histogram).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.