Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human ES cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, Alexa Fluor® 488 (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488 (Catalog #60069AD) (solid line histogram).

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of human ES cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, APC (filled histogram) or a mouse IgM, kappa APC isotype control antibody (solid line histogram).

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of human ES cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, PE (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (Catalog #60069PE) (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, PE. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, PE (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (solid line histogram).

Figure 4. Data for Unconjugated

(A) Flow cytometry analysis of human ES cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, followed by goat anti-mouse IgM, FITC (filled histogram), or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30 (Catalog #60069), followed by goat anti-mouse IgM, FITC (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human TRA-1-81, Clone TRA-1-81, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram). (D) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (lane 2) with Anti-Human TRA181 Antibody, Clone TRA-1-81.