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Mouse IgM, kappa Isotype Control Antibody, Clone MM­30

Mouse monoclonal IgM, kappa isotype control antibody

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Mouse IgM, kappa Isotype Control Antibody, Clone MM­30

Mouse monoclonal IgM, kappa isotype control antibody

From: 94 USD
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Mouse monoclonal IgM, kappa isotype control antibody
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Overview

The MM-30 antibody (IgM, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specfic antibody and indicated non-specific binding that may result from binding to Fc receptors or other cell components. The MM-30 antibody reportedly recognizes keyhole limpet hemocyanin.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Subtype
Isotype Controls
Target Antigen
IgM Isotype Control
Alternative Names
Not applicable
Conjugation
Alexa Fluor 488, PE, Unconjugated
Host Species
Mouse
Cell Type
Other
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
MM-30
Gene ID
Not applicable
Isotype
IgM, kappa

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human H1 embryonic stem (ES) cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC (solid line histogram), or a mouse IgM, kappa positive control antibody (Anti-Human TRA160 Antibody, Clone TRA-1-60R; Catalog #60064) followed by goat anti-mouse IgG, FITC (filled histogram). (B) Human H1 ES cells were cultured in mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgM, kappa positive control antibody (Anti-Human TRA-1-81 Antibody, Clone TRA-1-81; Catalog #60065).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human H1 embryonic stem (ES) cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488 (solid line histogram), or a mouse IgM, kappa positive control antibody (Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, Alexa Fluor® 488; Catalog #60064AD) (filled histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of human H1 embryonic stem (ES) cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (solid line histogram), or a mouse IgM, kappa positive control antibody (Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, PE; Catalog #60064PE) (filled histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Mouse IgM, kappa Isotype Control Antibody, Clone MM­30, PE
Catalog #
60069PE.1, 60069PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgM, kappa Isotype Control Antibody, Clone MM­30, Alexa Fluor® 488
Catalog #
60069AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgM, kappa Isotype Control Antibody, Clone MM­30
Catalog #
60069
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgM, kappa Isotype Control Antibody, Clone MM­30, PE
Catalog #
60069PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgM, kappa Isotype Control Antibody, Clone MM­30, PE
Catalog #
60069PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgM, kappa Isotype Control Antibody, Clone MM­30, Alexa Fluor® 488
Catalog #
60069AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgM, kappa Isotype Control Antibody, Clone MM­30
Catalog #
60069
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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