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Anti-Human CD11b Antibody, Clone ICRF44

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD11b

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Anti-Human CD11b Antibody, Clone ICRF44

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD11b

From: 153 USD
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Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD11b
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Overview

The ICRF44 antibody reacts with an extracellular epitope on CD11b (integrin αM), an ~170 kDa type 1 transmembrane glycoprotein which associates non-covalently with CD18 to form the heterodimeric Mac-1 receptor. Through its interactions with ligands such as ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, heparin, and fibrinogen, Mac-1 influences several processes, including the adherence of neutrophils and monocytes to stimulated endothelium, and phagocytosis of complement-coated particles. CD11b is expressed on the surface of granulocytes, monocytes, NK cells, dendritic cells, tissue macrophages, and subsets of T and B cells, and has been used as a marker to distinguish naïve and memory CD8+ T cells. CD11b is a relatively late marker for myeloid differentiation, and is undetectable on most myelomonocytic hematopoietic progenitor cells and more primitive cells. Certain mutations in CD11b give rise to the disorder systemic lupus erythematosus. The ICRF44 antibody reportedly inhibits leukocyte aggregation in response to the chemoattractant fMLP.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human Monocyte Enrichment Kit (Catalog #19059), and for labeling human mesenchymal cells grown in MesenCult™ Proliferation Kit (Human; Catalog #05411).
Subtype
Primary Antibodies
Target Antigen
CD11b
Alternative Names
C3biR, CR3, Integrin αM chain, Mac-1, MAC1, Mo1
Reactive Species
Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Human, Pig, Rhesus
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Myeloid Cells
Species
Human, Non-Human Primate, Other
Application
Cell Isolation, CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
ICRF44
Gene ID
3684
Isotype
IgG1, kappa

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD11b Antibody, Clone ICRF44, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD11b Antibody, Clone ICRF44, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD11b Antibody, Clone ICRF44, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti­Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD11b Antibody, Clone ICRF44, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD11b Antibody, Clone ICRF44, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD11b Antibody, Clone ICRF44, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD11b Antibody, Clone ICRF44, APC
Catalog #
60040AZ.1, 60040AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD11b Antibody, Clone ICRF44, FITC
Catalog #
60040FI.1, 60040FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD11b Antibody, Clone ICRF44, Alexa Fluor® 488
Catalog #
60040AD, 60040AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD11b Antibody, Clone ICRF44, PE
Catalog #
60040PE, 60040PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD11b Antibody, Clone ICRF44
Catalog #
60040
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD11b Antibody, Clone ICRF44, Biotin
Catalog #
60040BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD11b Antibody, Clone ICRF44, APC
Catalog #
60040AZ.1, 60040AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD11b Antibody, Clone ICRF44, FITC
Catalog #
60040FI.1, 60040FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD11b Antibody, Clone ICRF44, Alexa Fluor® 488
Catalog #
60040AD, 60040AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD11b Antibody, Clone ICRF44, PE
Catalog #
60040PE, 60040PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD11b Antibody, Clone ICRF44
Catalog #
60040
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD11b Antibody, Clone ICRF44, Biotin
Catalog #
60040BT
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (4)

Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Reliable Antibodies for Your Research
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Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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