Hematopoietic Stem and Progenitor Cell Research

The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.

The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaTox™ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).

Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.

Ongoing research on hematopoietic stem and progenitor cells (HSPCs) is directed toward the identification, isolation, and characterization of the primitive cell types that mediate rapid and/or sustained hematological recovery after cytoreductive therapy and transplantation. HSPCs are also being investigated in cell-based therapies for non-hematopoietic disorders.

The review below provides an overview of the current status of HSPC research with a focus on (i) assays used to detect and enumerate human and mouse stem and progenitor cells, (ii) phenotypic markers and methods used for their identification and isolation, and (iii) culture systems used to amplify stem and progenitor cells or to promote their differentiation in order to produce large numbers of mature blood cells for transfusion.

HSPC culture

Hematopoietic Stem and Progenitor Cell Isolation, Culture, and Assays

Read an overview of HSPC research with a focus on methods of isolation, culture, and assays used to detect and enumerate HSPCs.

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