StemSpan™

StemSpan™

Hematopoietic Cell Expansion Media and Supplements

StemSpan™ serum-free hematopoietic cell expansion media promote the expansion and/or lineage-specific differentiation of human hematopoietic stem and progenitor cells (HSPCs) when supplemented with hematopoietic growth factors and/or other stimuli selected by the user. StemSpan™ Expansion Supplements are pre-mixed cocktails of recombinant human cytokines and other additives formulated to selectively promote the expansion of CD34+ stem and progenitor cells, or to stimulate their differentiation into erythroid, myeloid or megakaryocyte progenitors, when added to StemSpan™ media.

Why Use StemSpan™ Media and Expansion Supplements?

  • Components are carefully selected to minimize lot-to-lot variability and support optimal and consistent culture results.
  • Media do not contain cytokines, allowing the flexibility to add StemSpan™ Expansion Supplements, cytokines and/or additives.
  • StemSpan™ SFEM II, combined with the appropriate Expansion Supplement, supports greater expansion of CD34+ cells and differentiation of erythroid cells and megakaryocytes than other media tested.
  • StemSpan™-ACF is the first commercially available animal component-free medium for culturing hematopoietic stem and progenitor cells.

Expansion Media

StemSpan™ SFEM

StemSpan™ SFEM

Formulation:

Serum-Free

Species:

  • Human
  • Mouse
  • Rat
  • Non-human primate

Recommended for:

Culture of HSPCs in serum-free conditions

Components:

  • BSA
  • Insulin
  • Tranferrin
  • Supplements
  • IMDM base

StemSpan™ SFEM II

StemSpan™ SFEM II

Formulation:

Serum-Free

Species:

Human

Recommended for:

Culture of HSPCs in serum-free conditions

Components:

  • BSA
  • Insulin
  • Tranferrin
  • Supplements
  • IMDM base

StemSpan™ H3000

StemSpan™ H3000

Formulation:

Xeno-Free

Species:

Human

Recommended for:

Culture of HSPCs in the absence of non-human animal-derived components

Components:

  • Human-derived and recombinant human proteins
  • IMDM base

ACF StemSpan™ Media

Formulation:

Animal-Component Free

Species:

Human

Recommended for:

Culture of HSPCs in the absence of human- or animal-derived components

StemSpan™-ACF
  • Expansion of CD34+ cells and differentiation of myeloid cells and megakaryocytes

StemSpan™ Erythroid Expansion Medium (ACF-E)
  • Differentiation of erythroid progenitor cells

Components:

  • Recombinant and synthetic components
  • IMDM base
Expand Details
Collapse Details


Expansion Supplements for Human HSPCs

Expansion of CD34+ Cells

Application:

Culture and expansion of human CD34+ hematopoietic cells

Differentiation into Erythroid Cells

Differentiation into Erythroid Cells

Application:

Expansion and lineage-specific differentiation of human CD34+ cells to generate large numbers of Glycophorin-A+ erythroblasts

Differentiation into Megakaryocytes

Differentiation into Megakaryocytes

Application:

Expansion and lineage-specific differentiation of CD34+ cells to generate large numbers of CD41+ megakaryocytes

Differentiation into Myeloid Cells

Differentiation into Myeloid Cells

Application:

Expansion and lineage-specific differentiation of CD34+ cells to generate large numbers of CD14+ and CD15+ myeloid cells
Expand Details
Collapse Details


Data

Figure 1. StemSpan™ SFEM II Serum-Free Expansion Medium Containing CC100 Cytokine Cocktail Supports Greater Expansion of Human CD34+ Cells Than Other Media Tested

Expansion of CD34+ cells normalized relative to the values obtained in StemSpan™ SFEM medium (dark gray bar) after culturing purified CD34+ CB cells for 7 days in StemSpan™ SFEM, SFEM II (gold bar) and ACF (orange bar), and six media from other commercial suppliers (light gray bars; Competitor 1-6, which included, in random order, StemLine II (Sigma), HPGM (Lonza), HP01 (Macopharma), SCGM (Cellgenix), StemPro34 (Life Technologies) and X-Vivo-15 (Lonza)). All media were supplemented with StemSpan™ CC100 Cytokine Cocktail. Vertical lines indicate 95% confidence limits, the range within which 95% of results fall. The numbers of cells produced in StemSpan™ SFEM II were significantly higher than in all other media, except the numbers of CB cells produced in StemSpan™-ACF (*p<0.05, paired t-test, n=6).



Figure 2. StemSpan™ SFEM II Serum-Free Expansion Medium Containing CD34+ Expansion Supplement Supports Greater Expansion of Human CD34+ Cells Than Other Media Tested

Expansion of CD34+ cells normalized relative to the values obtained in SFEM medium (dark gray bar) after culturing purified CD34+ CB cells for 7 days in StemSpan™ SFEM, SFEM II (gold bar) and ACF (orange bar), and six media from other suppliers (light gray bars; Competitor 1-6, which included, in random order, StemLine II (Sigma), HPGM (Lonza), HP01 (Macopharma), SCGM (Cellgenix), StemPro34 (Life Technologies) and X-Vivo-15 (Lonza)). All media were supplemented with the StemSpan™ CD34+ Expansion Supplement. Vertical lines indicate 95% confidence limits, the range within which 95% of results fall. The numbers of cells produced in StemSpan™ SFEM II were significantly higher than in all other media (*p<0.01, paired t-test, n=6).



Figure 3. StemSpan™ SFEM II Serum-Free Expansion Medium Containing Erythroid Expansion Supplement Supports Greater Expansion of Erythroid Cells Than Other Media Tested

The numbers of erythroid cells, normalized relative to the values obtained in StemSpan™ SFEM medium (dark gray bar), obtained after culturing purified CD34+ CB cells for 14 days in StemSpan™ SFEM, SFEM II (gold bar) and ACF (orange bar), and six media from other commercial suppliers (light gray bars; Competitor 1-6, which included, in random order, StemLine II (Sigma), HPGM (Lonza), HP01 (Macopharma), SCGM (Cellgenix), StemPro34 (Life Technologies) and X-Vivo-15 (Lonza)). All media were supplemented with StemSpan™ Erythroid Expansion Supplement. Vertical lines indicate 95% confidence limits, the range within which 95% of results fall. The numbers of cells produced in StemSpan™ SFEM II were significantly higher than in all other media (*p<0.05, paired t-test, n=6).



Figure 4. StemSpan™ SFEM II Serum-Free Expansion Medium Containing Megakaryocyte Expansion Supplement Supports Greater Expansion of Megakaryocytes Than Other Media Tested

The numbers of megakaryocytes, normalized relative to the values obtained in StemSpan™ SFEM medium (dark gray bar), obtained after culturing purified CD34+ CB cells for 14 days in StemSpan™ SFEM, SFEM II (gold bar) and ACF (orange bar), and six media from other commercial suppliers (light gray bars; Competitor 1-6, which included, in random order, StemLine II (Sigma), HPGM (Lonza), HP01 (Macopharma), SCGM (Cellgenix), StemPro34 (Life Technologies) and X-Vivo-15 (Lonza)). All media were supplemented with StemSpan™ Megakaryocyte Expansion Supplement (Catalog #02696). Vertical lines indicate 95% confidence limits, the range within which 95% of results fall. The numbers of cells produced in the StemSpan™ media were significantly higher than in the other media (*p<0.01 paired t-test, n=6).



Table 1. Production of Myeloid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement

Number of total nucleated cells (TNCs) produced per input human CB-derived CD34+ cell from 14 different donors and percentage of cells positive for myeloid markers CD13, CD14 and CD15 produced after 14-day culture in SFEM II containing Myeloid Expansion Supplement. Best expansion was obtained in SFEM II, but myeloid cell expansion may also be obtained in SFEM and ACF media. (*95% confidence limits (CL); the range within which 95% of the results will typically fall).



Key Applications

Ex Vivo Expansion of HSPCs for Rapid/Sustained Hematopoietic Recovery Post Transplantation

Generating Target Cells for Reprogramming to Make Induced Pluripotent Stem Cells

Gene Transfer into HSPCs

SFEM
Lechman et al. (2012) Attenuation of miR-126 Activity Expands HSC In Vivo without Exhaustion. Cell Stem Cell 11(6) SFEM II
Buechele C et al. (2015) MLL leukemia induction by genome editing of human CD34+ hematopoietic cells. Blood. Epub ahead of print
Copyright © 2016 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, AGGREWELL, CELLADHERE, CLONACELL, EASYPLATE, EASYSEP, ENDOCULT, EPICULT, ESCULT, ERYTHROCLEAR, FRESR, MAMMOCULT, MEGACULT, MESENCULT, METHOCULT, MYELOCULT, NEUROCULT, PNEUMACULT, PROSTACULT, RELESR, ROBOSEP, ROSETTESEP, SEPMATE, SMARTDISH, SPINSEP, STEMDIFF, STEMSEP, STEMSPAN, and STEMVISION are trademarks of STEMCELL Technologies Inc. All other trademarks and registered trademarksare the property of their respective holders.While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information.