StemSpan™-ACF Erythroid Expansion Medium
Animal component-free medium for culture and expansion of human erythroid cells
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StemSpan™-ACF Erythroid Expansion Medium (ACF-E) -
Label for StemSpan™-ACF Erythroid Expansion Medium (ACF-E)
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Overview
StemSpan™-ACF Erythroid Expansion Medium (ACF-E) is an animal component-free (ACF) medium for the in vitro culture and expansion of human hematopoietic cells. When supplemented with StemSpan™ Erythroid Expansion Supplement (100X; Catalog #02692), this medium supports the expansion and erythroid differentiation of human hematopoietic progenitor cells into CD71+ and Glycophorin A+ erythroblasts in ACF culture conditions. This medium only contains recombinant proteins and synthetic components; it does not contain serum or other human- or animal-derived components.
Advantages:
⦁ Animal component-free conditions specifically designed for expansion and differentiation of erythroid progenitor cells
⦁ Optimized for use with StemSpan™ Erythroid Expansion Supplement
⦁ Produces thousands of CD71+GlyA+ erythroid cells per input CD34+ cell
⦁ Optimized for use with StemSpan™ Erythroid Expansion Supplement
⦁ Produces thousands of CD71+GlyA+ erythroid cells per input CD34+ cell
Contains:
This product contains only recombinant proteins and synthetic components
Subtype:
Specialized Media
Cell Type:
Erythroid Cells; Hematopoietic Stem and Progenitor Cells
Species:
Human
Application:
Cell Culture; Differentiation; Expansion
Brand:
StemSpan
Area of Interest:
Stem Cell Biology; Transplantation Research
Formulation:
Animal Component-Free; Serum-Free
Related Products
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StemSpan™ Erythroid Expansion Supplement (100X)
Serum-free culture supplement for expansion of human erythroid cells -
Anti-Human CD235a (Glycophorin A) Antibody, Clone 2B7
Mouse monoclonal IgG1 antibody against human CD235a (glycophorin A) -
Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9
Mouse monoclonal IgG1 antibody against human CD71 (transferrin receptor)
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Data
Figure 1. Production of Erythroid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement
(A) Average numbers of erythroid cells generated after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM (black bars), SFEM II (grey bars), or StemSpan™-ACF Erythroid Expansion Medium (ACF-E, orange bars) media containing StemSpan™ Erythroid Expansion Supplement (Catalog #02692). Shown are the number of erythroid cells that express CD71 and/or Glycophorin A (GlyA) per input CD34+ cell. (B) The percentages of the different erythroid cell subsets generated in these cultures are shown, including CD71+GlyA+ erythroblasts, immature CD71+GlyA- erythroid progenitor cells and pro-erythroblasts, and CD71-/lowGlyA+ normoblasts. All three media supported the generation of thousands of erythroid cells per CB-derived CD34+ cell plated.
Table 1. Production of Erythroid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement
Yields and percentages of erythroid cells produced after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM, SFEM II or ACF-E media containing Erythroid Expansion Supplement. Erythroid cells were identified by flow cytometry after staining with antibodies against CD71 and GlyA. The % erythroid cells represent the percentage of cells that express CD71 and/or GlyA.
*CI: Confidence Interval.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.