• Optimized for use with StemSpan™ media. When combined with StemSpan™ SFEM II in particular, supports up to 4-fold higher expansion of erythroid cells from human CD34+ CB cells than other serum-free media on the market.
• Supplied as a 100X concentrate. After thawing and mixing, the tube contents can be added directly to any hematopoietic cell expansion medium of choice.
• Recombinant human interleukin 3 (IL-3)
• Recombinant human erythropoietin (EPO)
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Figure 1. CD71+GlyA+ Cells Produced from Cord Blood-Derived CD34+ Cells During 7 - 14 Days of Culture in StemSpan™ SFEM II Medium with Erythroid Expansion Supplement
Cord blood (CB)-derived CD34+ cells were cultured in StemSpan™ SFEM II containing Erythroid Expansion Supplement. After (A) 7 days and (B) 14 days, the cultured cells were stained with CD71 (Catalog #60106) and GlyA (Catalog #10423) antibodies and analyzed by flow cytometry. (A) During differentiation, the CD34+ cells progress to the CD71+GlyA+ erythroblast stage via CD71+GlyA- progenitors and pro-erythroblasts, which are still abundant on day 7. (B) With further culture these cells differentiate into erythroblasts and by day 14 most cells have become CD71+GlyA+. A small population of cells may mature further in these cultures into CD71-/loGlyA+ normoblasts and reticulocytes.
Figure 2. Production of Erythroid Cells from Human Cord Blood-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement
A) Average numbers of erythroid cells generated after culturing purified CD34+ cord blood (CB) cells (n=15) for 14 days in StemSpan™ SFEM (gray bars), SFEM II (gold bars) or ACF-E (brown bars) media containing Erythroid Expansion Supplement. Shown are the number of erythroid cells that express CD71 and/or GlyA per input CD34+ cell.
B) The percentages of the different erythroid cell subsets generated in these cultures are shown, including CD71-/loGlyA+ normoblasts, CD71+GlyA+ erythroblasts, and immature CD71+GlyA- erythroid progenitors and pro-erythroblasts. Vertical lines indicate 95% confidence limits (CL). Erythroid cell yields were significantly higher in SFEM II and ACF-E than in SFEM (p < 0.05;paired t-test, n=15). See table 1 for values and 95% confidence limits.
Table 1. Production of Erythroid Cells from Human Cord Blood-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement
Yields and percentages of erythroid cells produced after culturing purified CD34+ cord blood (CB) cells (n=15) for 14 days in StemSpan™ SFEM, SFEM II or ACF-E media containing Erythroid Expansion Supplement. Erythroid cells were identified by flow cytometry after staining with antibodies against CD71 and GlyA. The % erythroid cells represent the percentage of cells that express CD71 and/or GlyA. *CL: confidence limits.