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STEMvision™

Automated and Standardized Counting of CFU Assays of Human and Mouse Hematopoietic Progenitor Cells

STEMvision™ is a bench-top instrument and computer system designed specifically for automated imaging and counting of hematopoietic colonies in the colony-forming unit (CFU) assay. Instead of manually identifying and counting colonies using a microscope, the user simply loads a SmartDish™ culture plate containing up to 6 individual CFU assays in MethoCult™ medium into the automated CFU counter. The instrument captures an image of each 35 mm well, which is then analyzed using specialized software for each type of assay. By using an automated CFU counter to standardize colony counting, cord blood banks, transplant labs and research labs can ensure that their CFU assay results are accurate and reproducible.

Why Use STEMvision™?

  • Achieve more accurate colony counts than with manual counting.
  • Software reduces inter- and intra-individual and laboratory variation in colony counting.
  • Standardized software reduces the time it takes to train new students and staff to recognize and count colonies.
  • Culture wells are digitally imaged for permanent record keeping.

STEMvision™ for Automated CFU Counting of Human and Mouse CFU Assays

Human 7 Day

Cell Types:

Cord Blood

MethoCult™ Media:

Image Output:

Black and white images of entire 35 mm culture well and marked colonies

Data Output:

Total combined counts of all colonies

Imaging Time:

30 seconds

Analysis Time:

30 seconds

Human 14 Day

Cell Types:

  • Cord Blood
  • Bone Marrow
  • Mobilized Peripheral Blood

Image Output:

Color images highlighting hemoglobinized erythroid colonies with subclasses of colonies marked

Data Output:

Differential counts of colony subtypes (CFU-E, BFU-E, CFU-G/M/GM, CFU-GEMM)

Imaging Time:

1 minute

Analysis Time:

1 minute

Mouse 7-14 Day

Cell Types:

Bone Marrow

Image Output:

Black and white images of entire 35 mm culture well with 4 size classes of colonies marked

Data Output:

Total combined counts of all colonies in lineage-specific MethoCult™ media

Imaging Time:

30 seconds

Analysis Time:

30 seconds

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Data

Automated CFU counter, STEMvision™, images and analyses human CFU colonies

Figure 1. Representative STEMvision™ Images Showing Colonies Derived from Human Cord Blood Progenitors after 7 Days of Culture in MethoCult™ Express, and from Human Cord Blood, Bone Marrow and Mobilized Peripheral Blood Progenitors after 14 Days of Culture in MethoCult™ Optimum

These images have been analyzed by STEMvision™ Human (A) 7-Day and (B-D) 14-Day Analysis Packages. Green circles identify individual colonies in a 7-day cord blood CFU assay that scores total CFUs only (A). Orange and red circles identify erythroid colonies, yellow circles identify myeloid colonies and blue circles identify mixed colonies in 14-day cord blood (B), bone marrow (C) and mobilized peripheral blood (D) CFU assays. Erythroid and mixed colonies that contain hemoglobinized cells are shown in true red color.



Automated CFU counter, STEMvision™, images and analyses mouse CFU colonies

Figure 2. Representative STEMvision™ Images Showing Colonies Derived from Mouse Bone Marrow Progenitors after 12 Days of Culture in MethoCult™ GF M3434, MethoCult™ GF M3534 or MethoCult™ SF M3436 Media

Images of mouse bone marrow cells cultured in (A) MethoCult™ GF M3434, (B) MethoCult™ GF M3534 and (C) MethoCult™ SF M3436 were acquired using STEMvision™. The corresponding STEMvision™ Mouse Analysis Package (i.e. Total CFU Analysis Package (A), Myeloid CFU Analysis Package (B) or Erythroid CFU Analysis Package (C)) was used to analyze each image. Red circles identify the smallest colonies - size class 1, yellow circles - size class 2, blue circles - size class 3 and orange circles identify the largest colonies - size class 4.

Copyright © 2018 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, AGGREWELL, CELLADHERE, CLONACELL, EASYPLATE, EASYSEP, ENDOCULT, EPICULT, ESCULT, ERYTHROCLEAR, FRESR, MAMMOCULT, MEGACULT, MESENCULT, METHOCULT, MYELOCULT, NEUROCULT, PNEUMACULT, PROSTACULT, RELESR, ROBOSEP, ROSETTESEP, SEPMATE, SMARTDISH, SPINSEP, STEMDIFF, STEMSEP, STEMSPAN, and STEMVISION are trademarks of STEMCELL Technologies Canada Inc. All other trademarks and registered trademarks are the property of their respective holders. While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information.