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Protocol Resources for Colony-Forming Assays

Protocols

Colony-Forming Assays

One of the most widely used assays for characterizing hematopoietic stem and progenitor cells (HSPCs) is the colony-forming unit (CFU) assay, which allows measurement of the proliferation and differentiation potential of individual cells within a sample. Explore our collection of protocols and tech tips for information and support on setting up, optimizing, and standardizing the CFU assay.

Video How to Deplete Red Blood Cells from Small Volume Samples with HetaSep™
Video CFU Assay Instructions for Global Proficiency Testing Programs
Tech Tip Culture and Analysis of Hematopoietic Progenitor Cells in CFU Assays
Tech Tip Extending the Life of a CFU Assay
Tech Tip Colony-Forming Unit Assays with Mouse Cells
Protocol How to Calculate Cell Concentration for Plating CFU Assays Following RBC Clearance with HetaSep™
Video How to Set Up Hematopoietic Colony-Forming Unit (CFU) Assays
Technical Bulletin MethoCult™ for Rat Hematopoietic Colony-Forming Unit (CFU) Assays
Video How to Standardize and Automate Hematopoietic Colony Counting in CFU Assays with STEMvision™
Protocol How to Prepare and Plate Semi-Solid Methylcellulose Medium for Cell Culture
Video How to Set Up the CFU Assay for Custom Hematopoietic Training Courses Using SmartDish™
Tech Tip Maintaining Humidity in CFU Assays using MethoCult™ Media
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For detailed protocols developed specifically for our products, please consult the Product Information Sheet or enter the product keyword or catalog number below.


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