Detect & Quantify Hematopoietic Progenitor Cells in Colony-Forming Unit (CFU) Assays
MethoCult™ is a line of semi-solid methylcellulose-based media formulated to promote optimal growth and differentiation of hematopoietic progenitor cells in culture. MethoCult™ is the “Gold Standard” for the in vitro detection and quantification of hematopoietic progenitor cells in the
colony-forming unit (CFU)
or cell (CFC) assay, also known as the methylcellulose assay. A wide range of MethoCult™ media formulations are available for CFU assays with hematopoietic cells from human and mouse tissues. Select formulations can also be used with hematopoietic cells derived from human pluripotent stem cells (hPSCs) or from other species, specifically rats, non-human primates and dogs.
Serum-free, cytokine-free formulation suitable for testing sera or cytokines
Complete flexibility to add individual cytokines and supplements
Base methylcellulose 2.6% stock solution in Iscove’s MDM
Unsure Which MethoCult™ Formulation is Right for You?
For robust colony growth in the CFU assay, the most optimal MethoCult™ formulation will depend on your starting cell source. Use our Interactive Product Finder to determine which formulation is best for your experiment, and generate a complete materials list for your CFU assay.
Both STEMCELL and MethoCult™ grew out of the Media Preparation Service of the Terry Fox Laboratory for Hematology/Oncology Research at the British Columbia Cancer Agency. Now more than 20 years later, MethoCult™ is the most published methylcellulose-based medium for use in hematopoietic CFU assays.
CFU Assay Resources
Explore our resources for information and support on setting up, optimizing, and standardizing the CFU assay.