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STEMdiff™ Neural Organoid Maintenance Kit

Cell culture medium kit for long-term maintenance and maturation of patterned neural organoids

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STEMdiff™ Neural Organoid Maintenance Kit

Cell culture medium kit for long-term maintenance and maturation of patterned neural organoids

From: 350 USD
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Cell culture medium kit for long-term maintenance and maturation of patterned neural organoids
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Product Advantages


  • Reduce handling and media waste with fusion-free growth media

  • Obtain greater analytic sensitivity for disease phenotypes with reproducible morphology between lines and individual organoids

  • Eliminate matrix embedding steps with the matrix-free formulation and protocol

  • Easily process and section consistently sized organoids

  • Achieve long-term culture survival and reduced caspase-3 expression for neurotoxicity and neurodegenerative models

  • Combine modular region-patterned organoids to generate advanced AssemBloids™ for disease modeling and regenerative applications

What's Included

  • STEMdiff™ Neural Organoid Basal Medium 2, 500 mL
  • STEMdiff™ Neural Organoid Supplement A, 10 mL

Overview

Culture patterned brain organoids for extended periods (> 50 days) without the use of embedding matrices. With the essential components to prevent organoid fusion and retain a high yield of reproducible organoids, STEMdiff™ Neural Organoid Maintenance Kit is a serum-free cell culture medium designed for the maintenance and maturation of neural organoids adapted from protocols by Sergiu Paşca (F Birey et al. Nature, 2017). When used following STEMdiff™ Dorsal or Ventral Forebrain Organoid Differentiation Kits (Catalog #08620 and #08630), STEMdiff™ Neural Organoid Maintenance Kit allows for long-term organoid experiments for neurodegeneration research, biomarker discovery, and toxicity assays. The resulting brain-region-specific organoids have a cellular composition and structural organization that is representative of the developing human forebrain. Organoids generated with these kits can also be co-cultured as assembloids to study brain region interactions (F Birey et al. Nature, 2017).
Subtype
Specialized Media
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
Species
Human
Application
Characterization, Differentiation, Functional Assay, Immunofluorescence, Maintenance, Organoid Culture, Phenotyping, Spheroid Culture
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Organoids
Formulation Category
Serum-Free

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
STEMdiff™ Neural Organoid Maintenance Kit
Catalog #
100-0120
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
STEMdiff™ Neural Organoid Maintenance Kit
Catalog #
100-0120
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Neural Organoid Maintenance Kit
Catalog #
100-0120
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Neural Organoid Maintenance Kit
Catalog #
100-0120
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids
View All

Resources and Publications

Educational Materials (19)

STEMCELL Neural Product Portfolio
Brochure
STEMCELL Neural Product Portfolio
Organoids
Brochure
Organoids
Cryogenic Tissue Processing and Section Immunofluorescence of Neural Organoids
Protocol
Cryogenic Tissue Processing and Section Immunofluorescence of Neural Organoids
How to Dissociate 3D Neural Organoids into a Single-Cell Suspension
Protocol
How to Dissociate 3D Neural Organoids into a Single-Cell Suspension
Measuring Neuronal Activity in Human Pluripotent Stem Cell-Derived Cerebral Organoids on 3D High-Density Multi-Electrode Arrays
Protocol
Measuring Neuronal Activity in Human Pluripotent Stem Cell-Derived Cerebral Organoids on 3D High-Den...
How to Generate AssemBloids™ from hPSC-Derived Dorsal and Ventral Forebrain Organoid Co-Cultures
Protocol
How to Generate AssemBloids™ from hPSC-Derived Dorsal and Ventral Forebrain Organoid Co-Cultures
Building Three-Dimensional Human Brain Organoids
Wallchart
Building Three-Dimensional Human Brain Organoids
Derivation and Applications of Human Pluripotent Stem Cells
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
Cell-Reprogramming Technology and Neuroscience
Wallchart
Cell-Reprogramming Technology and Neuroscience
Using Human Pluripotent Stem Cell-Derived Neural Organoids for Disease Modeling
31:49
Webinar
Using Human Pluripotent Stem Cell-Derived Neural Organoids for Disease Modeling
ISSCR Innovation Showcase: Advanced Brain Organoid Co-Culture Systems
1:01:37
Webinar
ISSCR Innovation Showcase: Advanced Brain Organoid Co-Culture Systems
Challenges in Translating iPSC Technology
41:47
Webinar
Challenges in Translating iPSC Technology
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
50:49
Webinar
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
Guided Neural Organoid Culture Systems: Adding Dimensions to Model Neural Circuitry and Function
38:43
Webinar
Guided Neural Organoid Culture Systems: Adding Dimensions to Model Neural Circuitry and Function
Building Brain Organoids and AssemBloids™ to Study Human Development and Disease
1:02:03
Webinar
Building Brain Organoids and AssemBloids™ to Study Human Development and Disease
Nature Research Round Table: The Promise of Organoid Medicine
17:17
Webinar
Nature Research Round Table: The Promise of Organoid Medicine
Spotlight on Organoids: Expert Panel
Webinar
Spotlight on Organoids: Expert Panel
A Reliable, Efficient, and Feeder-Free Method to Generate Brain-Region-Specific Dorsal and Ventral Forebrain Organoids From Human Pluripotent Stem Cells to Model Early Human Brain Development
Scientific Poster
A Reliable, Efficient, and Feeder-Free Method to Generate Brain-Region-Specific Dorsal and Ventral F...
Dr. Bas Trietsch
Interview
Dr. Bas Trietsch

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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