Scale Up Your Cell Isolations

With the Easy 250 EasySep™ Magnet

Scale up your manual cell isolations and obtain cells from large-volume samples using the Easy 250 EasySep™ Magnet. Instead of splitting your cell suspension and performing multiple rounds of cell isolation, you can process samples of up to 225 mL and 12.5 x 109 cells in as little as 20 minutes with a single isolation.

The Easy 250 EasySep™ Magnet can be used with a standard T-75 cm² cell culture flask and EasySep™ reagents to speed up your cell isolations from large-volume samples, such as full-size leukopaks and whole blood. The isolated cells are immediately ready for downstream applications.

Why Use the Easy 250 Easysep™ Magnet?

  • Increase your cell isolation throughput by isolating cells from large-volume samples in a single isolation.
  • Isolate immune cells from sample volumes of up to 225 mL in as little as 20 minutes.
  • Achieve up to 99% cell purities of viable and functional cells.
  • Obtain your specific cell type of interest with compatible EasySep™ Cell Isolation Kits.

See the Easy 250 EasySep™ Magnet for yourself!

Save time when isolating immune cells from large-volume samples, such as full-size leukopaks, by using the Easy 250 EasySep™ Magnet.

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How Does the Easy 250 EasySep™ Magnet Work?

The Easy 250 EasySep™ Magnet is designed for fast and easy human cell isolation from leukopaks and whole blood. It uses column-free immunomagnetic EasySep™ cell isolation technology to process samples of up to 225 mL, or 12 billion cells, in a single step. The Easy 250 EasySep™ Magnet generates a magnetic field in the interior cavity that is strong enough to separate cells labeled with magnetic EasySep™ particles. With this design, the labeled cells are pulled to the sides of the tube when the sample is placed in the magnet, while untouched cells can be pipetted off into a new tube. The isolated cells are immediately ready for downstream applications.

Obtain purities of up to 99% for human cell types isolated from large sample volumes, including:

  • T cells and T cell subsets
  • B cells
  • Monocytes
  • Natural killer cells
  • Peripheral blood mononuclear cells
  • And more!

How Do You Use the Easy 250 EasySep™ Magnet?

Manual cell isolations from large-volume samples can be scaled up using the Easy 250 EasySep™ Magnet with a standard T-75 cm² cell culture flask and EasySep™ reagents.


Typical Easy 250 EasySep™ Magnet Cell Isolation Protocol (Negative Selection)

View the tech tip to learn the steps and key considerations when using T-75 cm² flasks with the Easy 250 EasySep™ Magnet.


How to Isolate Immune Cells from Large-Volume Samples Using the Easy 250 EasySep™ Magnet

Learn the key tips and considerations when using the Easy 250 EasySep™ Magnet for your large-scale immune cell isolations.

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STEMCELL Products for Manual Cell Isolation from Large-Volume Samples

Product
Catalog #
Easy 250 EasySep™ Magnet
EasySep™ Human T Cell Isolation Kit
EasySep™ Human CD3 Positive Selection Kit II
EasySep™ Human CD4+ T Cell Isolation Kit
EasySep™ Human CD4 Positive Selection Kit II
EasySep™ Human CD8+ T Cell Isolation Kit
EasySep™ Human CD8 Positive Selection Kit II
EasySep™ Human Monocyte Isolation Kit
EasySep™ Human CD14 Positive Selection Kit II
EasySep™ Direct Human PBMC Isolation Kit
EasySep™ RBC Depletion Reagent
EasySep™ Human NK Cell Isolation Kit
EasySep™ Human B Cell Isolation Kit

Try it in your own lab!

Unsure if the Easy 250 EasySep™ Magnet is right for your research? Try it in your own lab to isolate human immune cells from samples of up to 225 mL.

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