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EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

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EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

From: 1,190 USD
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Immunomagnetic cell isolation kit using particle release technology
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Product Advantages


  • Highly purified human CD4+CD127lowCD25+ Tregs isolated in less than 1 hour

  • No-wash removal of EasySep™ Releasable RapidSpheres™

  • Optional isolation of CD4+CD25- responder T cells from the same sample

What's Included

  • EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #18063)
    • EasySep™ Human CD25 Positive Selection Cocktail, 1 mL
    • EasySep™ Human CD127high Depletion Cocktail, 1 mL
    • EasySep™ Human CD4+ T Cell Enrichment Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • EasySep™ Release Buffer, 2 x 1 mL
  • EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #100-1136)
    • EasySep™ Human CD25 Positive Selection Cocktail (1 x 10 mL)
    • EasySep™ Human CD127high Depletion Cocktail (1 x 10 mL)
    • EasySep™ Human CD4+ T Cell Enrichment Cocktail (1 x 10 mL)
    • EasySep™ Releasable RapidSpheres™ 50201 (1 x 10 mL)
    • EasySep™ Dextran RapidSpheres™ 50103 (1 x 5 mL)
    • EasySep™ Release Buffer (Concentrated) (1 x 10 mL)
  • RoboSep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #18063RF)
    • EasySep™ Human CD25 Positive Selection Cocktail, 1 mL
    • EasySep™ Human CD127high Depletion Cocktail, 1 mL
    • EasySep™ Human CD4+ T Cell Enrichment Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • EasySep™ Release Buffer, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125) x 2
    • EasySep™ EasyTube™-14 (Catalog #20128)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

Easily isolate highly purified and magnetic particle-free human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples by immunomagnetic positive selection, with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are first labeled with antibody complexes recognizing CD25 and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles, which stay bound to the target cells, these RapidSpheres™ have a releasable feature. After separation using an EasySep™ magnet, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells using a release agent, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications. An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

For large-scale isolation of CD4+CD127lowCD25+ Tregs, from leukapheresis samples, see the large-format (1x1010 cells) kit (Catalog #100-1136).

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood CD4+CD25+CD127lowFOXP3+ T cells (Tregs), Frozen isolated with EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• RoboSep™-S (Catalog #21000)
• Easy 250 EasySep™ Magnet (Catalog #100-0821)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+, T Cells, Regulatory
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Typical Treg Isolation Using EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit Protocol

Figure 1. Typical EasySep™ Human Regulatory T Cell Isolation Profile When Processing Up to 1x 10^9 Cells Per Isolation

Starting with fresh or previously frozen PBMCs, the regulatory T cell content (CD4+CD25+FOXP3+) of the isolated fraction is typically 85.0 ± 4.8% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 1.8% and 88.2%, respectively.

EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit Protocol

EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit Protocol for the Separation of Tregs

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Catalog #
100-1136
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Catalog #
18063RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Catalog #
18063
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Catalog #
18063RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Catalog #
18063RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Catalog #
18063RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Catalog #
18063RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Catalog #
18063
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Catalog #
18063
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Catalog #
18063
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Catalog #
18063
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

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Quality Statement:

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