Figure 1. Growth of Human Pluripotent Stem Cells (hPSCs) in TeSR™-AOF 3D

TeSR™-AOF 3D supports the expansion and high viability of hPSCs over multiple passages in aggregate suspension culture. Shown are (A) cumulative viable cells, (B) daily fold expansion, and (C) end-of-passage viabilities in human ES cell lines (H1 and H7) and human iPS cell lines (WLS-1C and STiPS-F016) over 5 passages. Error bars represent ± SD, n = 3.

Figure 2. hPSCs Cultured in TeSR™-AOF 3D Express Markers of the Undifferentiated State and Differentiate to the Three Germ Layers

Shown are (A) OCT-4 and TRA-1-60 expression and (B) trilineage differentiation potential (ectoderm: % Nestin+/Pax6+, mesoderm: % Brach+, and endoderm: % Sox-17+/CXCR4+) using the STEMdiff™ Trilineage Differentiation Kit for multiple hPSC lines after 5 passages in TeSR™-AOF 3D. Error bars represent ± SD, n = 3.

Figure 3. Metabolic Profile of hPSCs During Culture in TeSR™-AOF 3D

(A) Glucose concentration in TeSR™-AOF 3D is maintained at optimal levels throughout the culture period. Dotted line represents the glucose level in TeSR™-AOF 3D Complete Seed Medium. (B) Lactate concentration is maintained at low levels (below 15 mM as indicated by the dotted line) throughout the culture period for multiple hPSC lines after 5 passages in TeSR™-AOF 3D. Error bars represent ± SD, n = 3.

Figure 4. Morphology of hPSC Aggregates in TeSR™-AOF 3D

Multiple hPSC lines maintained in TeSR™-AOF 3D depict morphology characteristic of hPSC aggregates in suspension culture, namely a mostly spherical shape, clear edges (not smooth or shiny), even color, a dimpled or pockmarked appearance, and are less than 400 µm in size.