Register: On-Demand Expansion of hPSCs in 3D Suspension Culture Course

Register now to complete a virtual training course on the expansion of human pluripotent stem cells (hPSCs) in 3D suspension culture.

Instructor Bio

Jane Savill - Manager, Scientific Support, Pluripotent Stem Cell Biology

Jane Savill is a Manager in the Scientific Support Department at STEMCELL Technologies. Jane obtained her PhD in Oncology from Cambridge University and prior to joining STEMCELL, she worked as a Product Development Scientist for a small start-up focused on Rheumatoid Arthritis diagnostics. At STEMCELL, Jane provides support with our product portfolios to the global customer base and sales staff. She specializes in STEMCELL’s hPSC product portfolio and also contributes as a trainer in the corporate and client training programs with a focus on hPSC products and protocols.

Course Curriculum

Session 1: Expansion of hPSCs in 3D Suspension Culture

Recognize the potential and key characteristics of hPSCs
Understand why researchers would require scaling up hPSC production
Be aware of the four key considerations for hPSC expansion

Mini Quiz: Test Your Knowledge

Estimated time for completion: 17 minutes

Session 2: Principles and Practices

Understand how to transition hPSCs from 2D to 3D suspension culture conditions
Understand the challenges of batch culturing and how fed-batch systems alleviate these issues
Examine the fed-batch protocol and cell characterization considerations
Be aware of the 3D suspension media offered by STEMCELL

Mini Quiz: Test Your Knowledge

Estimated time for completion: 20 minutes

Session 3: Scale Up: From Bottles to Bioreactors

Understand specific culture considerations for scaling-up, such as shear
Choose the recommended culture vessel for the appropriate volume for scaling-up 3D suspension cultures

Mini Quiz: Test Your Knowledge

Estimated time for completion: 8 minutes

Final Assessment

Complete this final assessment to test your knowledge and receive a certificate following successful completion.

Estimated time for completion: 15 minutes

The on-demand course is divided into 3 sessions, which should be reviewed sequentially. The sessions range from approximately 8 – 20 minutes in duration and the entire course, including the final assessment, can be completed in approximately 60 minutes. Note that this is not an accredited course and cannot be used to receive Continuing Education (CE) credits. If you wish to take an accredited course, please see our live virtual training.