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Culture patterned brain organoids for extended periods (> 50 days) without the use of embedding matrices. With the essential components to prevent organoid fusion and retain a high yield of reproducible organoids, STEMdiff™ Neural Organoid Maintenance Kit is a serum-free cell culture medium designed for the maintenance and maturation of neural organoids adapted from protocols by Sergiu Paşca (F Birey et al. Nature, 2017). When used following STEMdiff™ Dorsal or Ventral Forebrain Organoid Differentiation Kits (Catalog #08620 and #08630), STEMdiff™ Neural Organoid Maintenance Kit allows for long-term organoid experiments for neurodegeneration research, biomarker discovery, and toxicity assays. The resulting brain-region-specific organoids have a cellular composition and structural organization that is representative of the developing human forebrain. Organoids generated with these kits can also be co-cultured as assembloids to study brain region interactions (F Birey et al. Nature, 2017).
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Cell culture medium kit for robust generation of ventral forebrain neural organoids from human pluripotent stem cells
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STEMdiff™ Neural Organoid Maintenance Kit
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.