DisCoZyme 1, ACF

Animal component-free collagenase/dispase blend with high collagenase and high caseinase activity

DisCoZyme 1, ACF

Animal component-free collagenase/dispase blend with high collagenase and high caseinase activity

From: 268 USD
Catalog #
(Select a product)
Animal component-free collagenase/dispase blend with high collagenase and high caseinase activity
Add to Wish List

Product Advantages


  • Obtain cleaner cultures with an animal component-free solution

  • Dissociate tissues efficiently with a collagenase/dispase blend, high caseinase activity


Overview


Use DisCoZyme 1, Animal Component-Free (ACF) for tissue dissociation in applications where prevention of the introduction of potential animal-derived pathogens is necessary. This collagenase and dispase blend is sourced from clostridium histolyticum and bacillus polymyxa, respectively, and contains a minimum of 250 collagenase units/mg and 1000 caseinase units/mg of dry weight. Collagenase digests native collagen fibrils commonly found in connective tissues while the neutral amino-endoprotease, dispase, cleaves the N-terminal peptide bond of non-polar amino acid residues. The mild proteolytic activity of dispase allows it to maintain membrane integrity while isolating and passaging of primary cells. DisCoZyme 1, ACF is designed to contain high levels of collagenase and caseinase activity and has been used for the digestion of lung (Fujino et al.; Driscoll et al.) and neural tissues (Panchision et al.). Use DisCoZyme 2, ACF (Catalog #07450), if higher levels of caseinase activity are desired.
Subtype
Enzymatic
Alternative Names
Collagenase mix; Discozyme; Dispase mix; Collagenase/dispase mix; Neutral protease; Proteinase
Cell Type
Airway Cells, Neural Stem and Progenitor Cells
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Cell Culture
Area of Interest
Neuroscience, Respiratory Research
Molecular Weight
32 - 130 kDa

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
DisCoZyme 1, ACF
Catalog #
07449
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
DisCoZyme 1, ACF
Catalog #
07449
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.