Mobilized Human Peripheral Blood CD34+ Cells, Frozen

Primary human cells, frozen

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Mobilized Human Peripheral Blood CD34+ Cells, Frozen

Primary human cells, frozen

From: 1,190 USD

Mobilization

Size

1 x 10^6 cells 2 x 10^5 cells 5 x 10^6 cells 1 x 10^7 cells 2 x 10^7 cells

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Primary human cells, frozen

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Overview

Streamline your assays with ready-to-use, ethically sourced, mobilized primary CD34+ cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications.

Donors are mobilized with:
• G-CSF: a maximum of 10 µg/kg/day of granulocyte colony-stimulating factor for 3 - 5 days prior to collection (Catalog #70060)
• Plerixafor: a maximum of 0.24 mg/kg plerixafor for 1 day prior to collection (Catalog #70075)
• G-CSF and Plerixafor: a maximum of 10 µg/kg/day of G-CSF for 3 - 5 days prior to collection and a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection (Catalog #70073)

Isolated from leukapheresis samples using positive immunomagnetic separation techniques and cryopreserved in serum-free medium, cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above, after selecting from the product options. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.

Certain products are only available in select territories. Please contact your local sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

Browse our Frequently Asked Questions (FAQs) on Primary Cells.

• Serum-free cryopreservation medium
• 10% dimethyl sulfoxide (DMSO)

Frozen

Hematopoietic Stem and Progenitor Cells

Human

Peripheral Blood

Drug Discovery and Toxicity Testing

Normal

≥ 90% CD34+ (as a percentage of CD45+ cells) by flow cytometry

Data Figures

StemSpan™ HSC Plus Supplement Supports Expansion of Mobilized Peripheral Blood-Derived Primitive HSPCs Across Different StemSpan™ Media

Figure 1. StemSpan™ HSC Plus Supplement Supports Expansion of Mobilized Peripheral Blood-Derived Primitive HSPCs Across Different StemSpan™ Media

Purified CD34+ cells derived from G-CSF mobilized peripheral blood (mPB) were cultured at a concentration of 10,000 cells per mL in StemSpan™ media (SFEM, SFEM II, XF, and AOF). Cultures were supplemented with StemSpan™ CD34 Expansion Supplement alone (grey bars) or together with StemSpan™ HSC Plus Supplement (orange bars). After 7 days of culture, flow cytometry was used to analyze the (A) total nucleated cell (TNC) expansion, (B) expansion of CD34+ cell subsets, and (C) the frequency of CD34+ HSPC subsets: CD34+, CD34+CD45RA-CD90+ and CD34+CD45RA-CD90+EPCR+CD133+. Across all tested StemSpan™ media, StemSpan™ HSC Plus Supplement promoted greater expansion of CD34+CD45RA-CD90+ and CD34+CD45RA-CD90+CD133+EPCR+ cells compared to StemSpan™ CD34+ Expansion Supplement alone. Each subsequent cell subset was progressively more enriched for phenotypic stem/progenitor cells. Data shown are mean ± SEM (n=6).

CellPore™ Transfection System Enables Robust Knock-Out in HSPCs Derived from Mobilized Peripheral Blood Across a Wide Range of Cell Numbers

Figure 2. CellPore™ Transfection System Enables Robust Knock-Out in HSPCs Derived from Mobilized Peripheral Blood Across a Wide Range of Cell Numbers

Cryopreserved CD34+ HSPCs isolated from mobilized peripheral blood were cultured for 24 hours in complete StemSpan™ SFEM II culture medium. RNP complexes targeting the B2M gene were delivered to a range of cell numbers per reaction. Four days post-transfection, cell viability (A) and surface MHC-I marker expression, indicating editing efficiency (B) were measured via flow cytometry in bulk CD34+ HSPCs and primitive CD34+CD45RA-CD90+ HSCs. Comparable viability and editing efficiency were obtained across the cell number range tested. Data are shown as mean ± SD, n = 2 - 5.

Figure 3. Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells Generate Hematopoietic Colonies in CFU Assays

Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells (Catalog #70060) were thawed, plated at a concentration of 5 x 10^2 viable cells/dish and cultured in MethoCult™ Optimum Medium (Catalog #04034) for 14 days. Shown are colonies produced by hematopoietic progenitor cells in the sample, imaged with STEMvision™.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

G-CSF and Plerixafor Mobilized Human Peripheral Blood CD34+ Cells, Frozen

Catalog #

70073, 70073.4, 70073.1, 70073.3

Lot #

All

Language

English

Document Type

Product Information Sheet

Product Name

G-CSF Mobilized Human Peripheral Blood CD34+ Cells, Frozen

Catalog #

70060.3, 70060.1, 70060

Lot #

All

Language

English

Document Type

Product Information Sheet

Product Name

Plerixafor Mobilized Human Peripheral Blood CD34+ Cells, Frozen

Catalog #

70075.3, 70075.2, 70075, 70075.1

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for Human Pluripotent Stem Cell Research

Reprogramming

Cell Engineering

Maintenance

Differentiation

Workflow Stages for Hematopoietic Stem and Progenitor Cell Research

Cell Sourcing and Isolation

Cell Engineering

Expansion and Differentiation

Analysis

Workflow Stages for Hematopoietic Cell Therapy Development

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Workflow Stages for In Vitro Circulatory System Tools for NAMs

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Resources and Publications

Related Products

Save on high-quality cells! Take advantage of our bulk pricing discounts and price matching offer to get the best value for your purchase. Browse our Frequently Asked Questions for more information.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

Mobilized Human Peripheral Blood CD34+ Cells, Frozen

Primary human cells, frozen

Mobilized Human Peripheral Blood CD34+ Cells, Frozen

Primary human cells, frozen

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Overview

Data Figures

Protocols and Documentation

Applications

Resources and Publications

Educational Materials (13)

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