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Mobilized Human Peripheral Blood CD34+ Cells, Frozen

Primary human cells, frozen
From: 347 USD

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Primary human cells, frozen
From: 347 USD

Overview

Primary human CD34+ cells were isolated from mobilized peripheral blood (PB) leukapheresis samples using positive immunomagnetic separation techniques. PB was collected using acid citrate dextrose solution A (ACDA) as an anticoagulant. Donors were mobilized with:
• Catalog #70060: a maximum of 10 µg/kg/day of granulocyte colony-stimulating factor (G-CSF) for 3 - 5 days prior to collection
• Catalog #70075: a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection
• Catalog #70073: a maximum of 10 µg/kg/day of G-CSF for 3 - 5 days prior to collection and a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection

Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.

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Contains:
• Serum-free cryopreservation medium
• 10% dimethyl sulfoxide (DMSO)
Subtype:
Frozen
Cell Type:
Hematopoietic Stem and Progenitor Cells
Species:
Human
Cell and Tissue Source:
Peripheral Blood
Donor Status:
Normal
Purity:
Purity of CD34+ cells is ≥ 90% by flow cytometry as a percentage of CD45+ cells

Scientific Resources

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells Generate Hematopoietic Colonies in CFU Assays

Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells (Catalog #70060) were thawed, plated at a concentration of 5 x 10^2 viable cells/dish and cultured in MethoCult™ Optimum Medium (Catalog #04034) for 14 days. Shown are colonies produced by hematopoietic progenitor cells in the sample, imaged with STEMvision™.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.