Mobilized Human Peripheral Blood CD34+ Cells, Frozen

Primary human cells, frozen
Catalog #
70060_C
Primary human cells, frozen
From: 394 USD

Overview

Streamline your assays with ready-to-use, ethically sourced, mobilized primary CD34+ cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications.

Donors are mobilized with:
• G-CSF: a maximum of 10 µg/kg/day of granulocyte colony-stimulating factor for 3 - 5 days prior to collection (Catalog #70060)
• Plerixafor: a maximum of 0.24 mg/kg plerixafore for 1 day prior to collection (Catalog #70075)
• G-CSF and Plerixafor: a maximum of 10 µg/kg/day of G-CSF for 3 - 5 days prior to collection and a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection (Catalog #70073)

Isolated from leukapheresis samples using positive immunomagnetic separation techniques and cryopreserved in serum-free medium, cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above, after selecting from the product options. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.

Certain products are only available in select territories. Please contact your local sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

Browse our Frequently Asked Questions (FAQs) on Primary Cells.
Contains
• Serum-free cryopreservation medium
• 10% dimethyl sulfoxide (DMSO)
Subtype
Frozen
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Cell and Tissue Source
Peripheral Blood
Donor Status
Normal
Purity
Purity of CD34+ cells is ≥ 90% by flow cytometry as a percentage of CD45+ cells

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
G-CSF Mobilized Human Peripheral Blood CD34+ Cells, Frozen
Catalog #
70060, 70060.1, 70060.2, 70060.3, 70060.4
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
G-CSF and Plerixafor Mobilized Human Peripheral Blood CD34+ Cells, Frozen
Catalog #
70073, 70073.1, 70073.2, 70073.3, 70073.4
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Plerixafor Mobilized Human Peripheral Blood CD34+ Cells, Frozen
Catalog #
70075, 70075.1, 70075.2, 70075.3, 70075.4
Lot #
All
Language
English

Educational Materials (11)

Brochure
STEMvision™ Automated and Standardized Counting of CFU Assays of Human Hematopoietic Cells
Brochure
Human Primary Cells
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays
Wallchart
Human Hematopoietic Stem and Progenitor Cell Phenotyping
Wallchart
Identification of Colonies Derived from Human Hematopoietic Progenitors
Video
How to Thaw Frozen Human Primary Cells
6:05
How to Thaw Frozen Human Primary Cells
Webinar
Optimization and Standardization of the Colony-Forming Unit Assay for Hematopoietic Progenitor Cells
53:35
Optimization and Standardization of the Colony-Forming Unit Assay for Hematopoietic Progenitor Cells
Webinar
Humanized Mouse Models for Hematopoietic Stem Cell Research: Principles and Pitfalls
50:50
Humanized Mouse Models for Hematopoietic Stem Cell Research: Principles and Pitfalls
Webinar
Identification of Stem Cells in Myeloid Malignancies - Opportunities for Discovery and Translation
58:45
Identification of Stem Cells in Myeloid Malignancies - Opportunities for Discovery and Translation
Mini Review
Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells Generate Hematopoietic Colonies in CFU Assays

Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells (Catalog #70060) were thawed, plated at a concentration of 5 x 10^2 viable cells/dish and cultured in MethoCult™ Optimum Medium (Catalog #04034) for 14 days. Shown are colonies produced by hematopoietic progenitor cells in the sample, imaged with STEMvision™.

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