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Data and Publications
(A) The biological activity of Human Recombinant TGF-beta 3 was tested by its ability to inhibit proliferation of HT-2 cells in the presence of IL-4. Inhibition of cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation inhibition is at 50% of maximum. The EC50 in the example above is 0.0445 ng/mL. (B) 1 μg of Human Recombinant TGF-beta 3 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.