Hydrocortisone

Enhances proliferation and differentiation of endothelial, epithelial, mesenchymal, and oligodendrocyte cells

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Enhances proliferation and differentiation of endothelial, epithelial, mesenchymal, and oligodendrocyte cells
From: 50 USD

Overview

Hydrocortisone is a glucocorticoid hormone with anti-inflammatory and immunosuppressive effects (Kang et al.; Langhoff & Ladefoged). It is also commonly used as a supplement for endothelial, epithelial, mesenchymal, or oligodendrocyte cell culture media, as it supports growth and differentiation. This product is supplied as the sodium salt of hydrocortisone 21-hemisuccinate, which is a more water-soluble form of hydrocortisone.

Hydrocortisone is required as a supplement in MyeloCult™ H5100 (Human; Catalog #05100) and MyeloCult™ M5300 (Mouse; Catalog #05300), for long-term cultures and the long-term culture-initiating cell (LTC-IC) assay. For complete instructions, refer to the Technical Manual for Human (Document #28412) or Mouse (Document #28417) LTC-IC Assays, available at www.stemcell.com or contact us to request a copy.

MAINTENANCE AND SELF-RENEWAL
· Enhances proliferation and self-renewal of limbal stem cells in explant cultures (Yu et al.).
· Supports long-term growth of immature human myeloid cells in culture (Salahuddin et al.).
· Increases the activity of glucose 6-phosphate dehydrogenase in mouse mammary epithelial cells in culture in the presence of insulin (Oka & Perry).

DIFFERENTIATION
· With ATRA, EGF, KGF, and HGF, promotes epithelial differentiation of adipose-derived stem cells (Li et al.).
· Promotes differentiation of mouse embryonic stem (ES) cell-derived definitive endoderm toward lung alveolar epithelial cells (Mokhber Dezfouli et al.).

IMMUNOLOGY
· Inhibits the bioactivity of the proinflammatory cytokine interleukin 6 (IC₅₀ = 6.7 mM; Kang et al.).
·Suppresses the activity on phytohemagglutinin (PHA)-stimulated lymphocytes in vitro (Langhoff & Ladefoged).
Alternative Names:
Cortisol 21-hemisuccinate; Hydrocortisone sodium succinate; ST51037292; U-4905
CAS Number:
125-04-2
Chemical Formula:
C₂₅H₃₃O₈ • Na
Molecular Weight:
484.5 g/mol
Purity:
≥ 98%

Scientific Resources

Product Documentation

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Educational Materials

(5)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Publications

(1)
Cell stem cell 2020

Restraining Lysosomal Activity Preserves Hematopoietic Stem Cell Quiescence and Potency.

R. Liang et al.

Abstract

Quiescence is a fundamental property that maintains hematopoietic stem cell (HSC) potency throughout life. Quiescent HSCs are thought to rely on glycolysis for their energy, but the overall metabolic properties of HSCs remain elusive. Using combined approaches, including single-cell RNA sequencing (RNA-seq), we show that mitochondrial membrane potential (MMP) distinguishes quiescent from cycling-primed HSCs. We found that primed, but not quiescent, HSCs relied readily on glycolysis. Notably, in vivo inhibition of glycolysis enhanced the competitive repopulation ability of primed HSCs. We further show that HSC quiescence is maintained by an abundance of large lysosomes. Repression of lysosomal activation in HSCs led to further enlargement of lysosomes while suppressing glucose uptake. This also induced increased lysosomal sequestration of mitochondria and enhanced the competitive repopulation ability of primed HSCs by over 90-fold in vivo. These findings show that restraining lysosomal activity preserves HSC quiescence and potency and may be therapeutically relevant.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.