This kit contains EpiCult™-B Basal Medium (Human) and EpiCult™-B Proliferation Supplement (Human). Addition of Hydrocortisone Stock Solution (Catalog #07925) is required.
- EpiCult™-B Basal Medium (Human), 100 mL
- EpiCult™-B Proliferation Supplement (Human), 1 mL
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Figure 1. Protocol for isolation and identification of human and mouse mammary epithelial progenitor cells
Phase contrast photographs of (A) a pure human myoepithelial cell colony, (B) a pure human luminal cell colony, and (C) a mixed human colony. (D) is a mouse colony. Unlike human mammary CFC colonies, subtypes of mouse mammary epithelial cell colonies are not easily identifiable. All colonies were cultured in either EpiCult®-B (Human: Catalog #05601) or EpiCult®-B (Mouse:Catalog #5610) in the presence of an irradiated NIH 3T3 feeder layer. Colonies were visualized by staining with Wright"s Giemsa. (E) is a picture of mammospheres obtained from primary human mammary epithelial cells and (F) is an image of tumorspheres obtained from MCF7 human breast cancer cell line.