Human Recombinant PDGF-AA

Platelet-derived growth factor AA

Human Recombinant PDGF-AA

Platelet-derived growth factor AA

From: 189 USD
Catalog #
78095_C
Platelet-derived growth factor AA

Overview

Platelet-derived growth factor (PDGF) is a dimeric glycoprotein consisting of two disulfide bridge-stabilized polypeptide chains, A and B, which are assembled as heterodimers (PDGF-AB) or homodimers (PDGF-AA and PDGF-BB) (Fretto et al.; Westermark & Heldin). PDGF signals through the receptor tyrosine kinases PDGFRalpha and PDGFRbeta. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src, and PI3K/AKT (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin, such as fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). It has been suggested that PDGF-AA is an important autocrine regulator of vascular endothelial growth factor (VEGF) expression in non-small cell lung carcinomas (Shikada et al.). PDGF-AA also mediates proliferation of oligodendrocyte progenitor cells and oligodendrocyte lineage differentiation through the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) (Hu et al.). PDGF-AA is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into oligodendrocyte precursor cells (Piao et al.).
Subtype
Cytokines, Growth Factors
Alternative Names
GDGF, Glioma-derived growth factor, ODGF, Osteosarcoma-derived growth factor, Platelet-derived growth factor-AA
Cell Type
Mesenchymal Cells, PSC-Derived, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons, Other
Species
Human
Area of Interest
Neuroscience, Stem Cell Biology
Molecular Weight
14.4 kDa monomer; 28.9 kDa dimer
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant PDGF-AA was tested by its ability to promote the proliferation of BALB/c 3T3 cells. Cell proliferation was measured after 46 hours using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 21 ng/mL. (B) 1 μg of Human Recombinant PDGF-AA was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant PDGF-AA is a homodimer of 14.4 kDa subunits with a predicted total molecular mass of 28.9 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78095, 78095.1, 78095.3
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78095, 78095.1, 78095.3
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (1)

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