Human Recombinant PDGF-AA

Platelet-derived growth factor AA

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Platelet-derived growth factor AA
From: 202 USD

Overview

Platelet-derived growth factor (PDGF) is a dimeric glycoprotein consisting of two disulfide bridge-stabilized polypeptide chains, A and B, which are assembled as heterodimers (PDGF-AB) or homodimers (PDGF-AA and PDGF-BB) (Fretto et al.; Westermark & Heldin). PDGF signals through the receptor tyrosine kinases PDGFRalpha and PDGFRbeta. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src, and PI3K/AKT (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin, such as fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). It has been suggested that PDGF-AA is an important autocrine regulator of vascular endothelial growth factor (VEGF) expression in non-small cell lung carcinomas (Shikada et al.). PDGF-AA also mediates proliferation of oligodendrocyte progenitor cells and oligodendrocyte lineage differentiation through the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) (Hu et al.). PDGF-AA is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into oligodendrocyte precursor cells (Piao et al.).
Alternative Names:
GDGF, Glioma-derived growth factor, ODGF, Osteosarcoma-derived growth factor, Platelet-derived growth factor-AA
Cell Type:
Mesenchymal Cells, PSC-Derived; Neural Cells, PSC-Derived; Neural Stem and Progenitor Cells; Neurons; Other
Species:
Human
Area of Interest:
Neuroscience; Stem Cell Biology
Molecular Weight:
14.4 kDa monomer; 28.9 kDa dimer
Purity:
≥ 95%

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(1)

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Data and Publications

Data

(A) The biological activity of Human Recombinant PDGF-AA was tested by its ability to promote the proliferation of BALB/c 3T3 cells. Cell proliferation was measured after 46 hours using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 21 ng/mL. (B) 1 μg of Human Recombinant PDGF-AA was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant PDGF-AA is a homodimer of 14.4 kDa subunits with a predicted total molecular mass of 28.9 kDa.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.