Human Recombinant M-CSF

Macrophage colony-stimulating factor

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Macrophage colony-stimulating factor
From: 196 USD

Overview

Macrophage colony-stimulating factor (M-CSF) is a homodimeric glycoprotein growth factor that regulates proliferation and differentiation of myeloid hematopoietic progenitor cells to mononuclear phagocytic cell lineages, including monocytes, macrophages, and osteoclasts. M-CSF is a crucial factor for the development of tissue-resident macrophages in most tissues (Ginhoux & Jung). It is required for the maturation and activation of monocytes and macrophages, and regulates inflammatory responses in conjunction with other stimuli such as IFN-γ, LPS, and IL-4 (Murray et al.). M-CSF is also required for bone resorption by osteoclasts, and is involved in the development and regulation of the placenta, mammary gland, and brain. M-CSF is produced by monocytes, fibroblasts, osteoclasts, stromal cells, endothelial cells, and tumor cells (Chockalingam & Ghosh).

M-CSF exerts its biological effects by signaling through a receptor tyrosine kinase (CSF-1R or M-CSF-R) encoded by the c-fms proto-oncogene (Hamilton). CSF-1R shares similar structural features with other growth factor receptors, including the stem cell factor (SCF) receptor, platelet-derived growth factor receptor (PDGF-R), and Flt3/Flk-2 receptor tyrosine kinase. Stimulation of the CSF-1R upon binding to M-CSF activates MAPK, PI3K, and PLCγ signaling pathways (Chockalingam & Ghosh). Human and mouse M-CSF sequences are highly conserved both at nucleotide and amino acid levels (80% homology; DeLamarter et al.).
Alternative Names:
Colony stimulating factor 1, CSF-1
Cell Type:
Hematopoietic Stem and Progenitor Cells; Dendritic Cells; Monocytes; Myeloid Cells; Neurons; Mesoderm, PSC-Derived
Species:
Human
Area of Interest:
Stem Cell Biology; Immunology
Molecular Weight:
18.5 kDa monomer; 37.1 kDa dimer
Purity:
≥ 95%

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Data and Publications

Data

(A) The biological activity of Human Recombinant M-CSF was tested by its ability to promote the proliferation of NFS-60 cells. Cell proliferation was measured after 44 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.9 - 1.4 ng/mL.
(B) 1 μg of Human Recombinant M-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant M-CSF is a homodimer of 18.5 kDa subunits with a predicted total molecular mass of 37.1 kDa.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.