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Anti-Human CD36 Antibody, Clone FA6-152

Mouse monoclonal IgG1 antibody against human, rat CD36

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Anti-Human CD36 Antibody, Clone FA6-152

Mouse monoclonal IgG1 antibody against human, rat CD36

From: 270 USD
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Mouse monoclonal IgG1 antibody against human, rat CD36
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Overview

The FA6-152 antibody reacts with CD36, an ~88 kDa transmembrane glycoprotein that functions as a scavenger receptor, cell adhesion molecule, and signal transducer on the surface of many types of cells including platelets, monocytes, macrophages, erythrocyte precursors, endothelial and epithelial cells, and some macrophage-derived dendritic cells. The topology of the protein is thought to comprise a large extracellular loop with the N- and C-termini both inserted through the cell membrane. CD36 binds multiple ligands such as thrombospondin, collagen, lipoproteins, and long-chain fatty acids, and has evident roles in the phagocytotic clearance of apoptotic cells, inhibition of angiogenesis, metabolism of glucose and fatty acids, inflammation, and the pathogenesis of malaria. Binding of the FA6-152 antibody to CD36 reportedly blocks its interaction with thrombospondin, collagen, apoptotic cells, and modified LDL, and induces agglutination of fetal but not adult erythrocytes. The epitope for FA6-152 reportedly resides within an immunodominant domain of CD36 comprising amino acids 155 - 183.
Subtype
Primary Antibodies
Target Antigen
CD36
Alternative Names
GP88, GPIIIb, GPIV, FAT, platelet glycoprotein 4, platelet glycoprotein IIIb, platelet glycoprotein IV, SCARB3, thrombospondin receptor
Reactive Species
Human, Rat
Conjugation
FITC, Unconjugated
Host Species
Mouse
Cell Type
Monocytes, Other
Species
Human, Rat
Application
Cell Isolation, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Neutralization and Blocking, Western Blotting
Area of Interest
Immunology
Clone
FA6-152
Gene ID
948
Isotype
IgG1, kappa

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD36 Antibody, Clone FA6-152, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram). (B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD36 Antibody, Clone FA6-152, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for FITC-Conjugated

Figure 2. Data for FITC-Conjugated

(A) Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD36 Antibody, Clone FA6-152, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD36 Antibody, Clone FA6-152, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram). (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD14 Positive Selection Kit and labeled with Anti-Human CD36 Antibody, Clone FA6-152, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD36 Antibody, Clone FA6-152
Catalog #
60084
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD36 Antibody, Clone FA6-152, FITC
Catalog #
100-1577
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD36 Antibody, Clone FA6-152
Catalog #
60084
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD36 Antibody, Clone FA6-152, FITC
Catalog #
100-1577
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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