Human/Mouse Recombinant BMP-2, ACF

Bone morphogenetic protein 2, animal component-free
Catalog #
78135_C
Bone morphogenetic protein 2, animal component-free
From: 204 USD

Overview

Bone morphogenetic protein 2 (BMP-2) is a member of the transforming growth factor beta (TGF-β) superfamily. BMP-2 is a disulfide-linked homodimer, acts as a ligand for complexes of type I and II BMP receptors, and primarily activates SMAD1/5/8 signaling (Nohe et al.). BMP-2 is a potent differentiation factor and directs human pluripotent stem cells (hPSCs) towards various cell types including extra-embryonic endoderm, mesenchymal cells, and chondrocytes (Pera et al.). Although BMP-2 expression is low in healthy cartilage, its expression is upregulated at the site of cartilage damage (Blaney Davidson et al.). BMP-2 induces bone and cartilage formation in vitro and is able to induce chondrogenesis in human mesenchymal stem cells (Schmitt et al.). This product is animal component-free.
Subtype
Cytokines
Alternative Names
BDA2, BMP-2A, BMP2, Bone morphogenetic protein 2A
Cell Type
Endoderm, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Pluripotent Stem Cells
Species
Human, Mouse
Area of Interest
Epithelial Cell Biology, Neuroscience, Stem Cell Biology
Molecular Weight
13 kDa monomer; 26.1 kDa dimer
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human/Mouse Recombinant BMP-2, ACF
Catalog #
78135
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human/Mouse Recombinant BMP-2, ACF
Catalog #
78135
Lot #
All
Language
English

Educational Materials (9)

Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Products for Human Pluripotent Stem Cells
Technical Bulletin
Weekend-Free Culture of Human Pluripotent Stem Cells in mTeSR™1 or TeSR™-E8™
Wallchart
Identification of Colonies Derived from Human Hematopoietic Progenitors
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Stem Cell States: Naive to Primed Pluripotency
Webinar
New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells
1:06:52
New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells
Webinar
Methods and Culture Reagents to Maintain a Spectrum of hPSC States from Naive-Like to Primed
23:35
Methods and Culture Reagents to Maintain a Spectrum of hPSC States from Naive-Like to Primed
Mini Review
Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human/Mouse Recombinant BMP-2, ACF was tested by its ability to induce alkaline phosphatase activity in ATDC-5 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase production is at 50% of maximum. The EC50 in the example above is 74 ng/mL. (B) 1 μg of Human/Mouse Recombinant BMP-2, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

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