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Data and Publications
(A) The biological activity of Human Recombinant BMP-2, ACF was tested by its ability to induce alkaline phosphatase activity in ATDC-5 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase production is at 50% of maximum. The EC50 in the example above is 74 ng/mL. (B) 1 μg of Human Recombinant BMP-2, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.