EasySep™ Human Myeloid Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit
Catalog #
17893_C
Immunomagnetic positive selection cell isolation kit
From: 695 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human Myeloid Positive Selection Kit II is designed to isolate CD33+ and CD66b+ cells from ammonium chloride lysed blood or bone marrow by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33, CD66b and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 and CD66b antigens are expressed on myeloid progenitors monocytes and granulocytes.


This product replaces the EasySep™ Human Myeloid Positive Selection Kit (Catalog #18653) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components
  • EasySep™ Human Myeloid Positive Selection Kit II (Catalog #17893)
    • EasySep™ Human Myeloid Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
  • RoboSep™ Human Myeloid Positive Selection Kit II with Filter Tips (Catalog #17893RF)
    • EasySep™ Human Myeloid Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets, Myeloid Cells
Species
Human
Sample Source
PMNC, Whole Blood
Selection Method
Positive
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Myeloid Positive Selection Kit II
Catalog #
17893
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Myeloid Positive Selection Kit II
Catalog #
17893RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Myeloid Positive Selection Kit II
Catalog #
17893
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Myeloid Positive Selection Kit II
Catalog #
17893
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Myeloid Positive Selection Kit II
Catalog #
17893
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Myeloid Positive Selection Kit II
Catalog #
17893RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Myeloid Positive Selection Kit II
Catalog #
17893RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Myeloid Positive Selection Kit II
Catalog #
17893RF
Lot #
All
Language
English

Educational Materials(6)

Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with peripheral blood MNCs, the myeloid cell content (CD14+CD66b+) of the isolated fraction is typically 96.5 ± 1.3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 59.2% and 94.6%, respectively.

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