EasySep™ Human Myeloid Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit

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From: 695 USD

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Immunomagnetic positive selection cell isolation kit
From: 695 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human Myeloid Positive Selection Kit II is designed to isolate CD33+ and CD66b+ cells from ammonium chloride lysed blood or bone marrow by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33, CD66b and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 and CD66b antigens are expressed on myeloid progenitors monocytes and granulocytes.


This product replaces the EasySep™ Human Myeloid Positive Selection Kit (Catalog #18653) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components:
  • EasySep™ Human Myeloid Positive Selection Kit II (Catalog #17893)
    • EasySep™ Human Myeloid Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
  • RoboSep™ Human Myeloid Positive Selection Kit II with Filter Tips (Catalog #17893RF)
    • EasySep™ Human Myeloid Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Granulocytes and Subsets; Myeloid Cells
Species:
Human
Sample Source:
PMNC; Whole Blood
Selection Method:
Positive
Brand:
EasySep; RoboSep
Area of Interest:
Chimerism; HLA; Immunology

Scientific Resources

Product Documentation

Educational Materials

(6)
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Myeloid Cell Research
Cell Sourcing
Cell Isolation
Cell Characterization
Chimerism Analysis
Cell Isolation
Characterization

Data and Publications

Data

Starting with peripheral blood MNCs, the myeloid cell content (CD14+CD66b+) of the isolated fraction is typically 96.5 ± 1.3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 59.2% and 94.6%, respectively.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.