EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit

Immunomagnetic positive selection cell isolation kit
Catalog #
17884_C
Immunomagnetic positive selection cell isolation kit
From: 715 USD
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Required Products
  1. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

  2. "The Big Easy" EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

Overview

Isolate highly purified CD33+ and CD66b+ myeloid cells, including myeloid progenitor cells, granulocytes, and monocytes, from fresh whole blood prior to chimerism testing.

Desired cells are targeted with tetrameric antibody complexes recognizing CD33, CD66b, and dextran-coated magnetic particles, and separated without the use of columns through immunomagnetic positive selection using an EasySep™ magnet. Cells of interest remain in the tube while unwanted cells are poured off.

This product has replaced the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA) for faster cell isolations. For even higher efficiency, this product can be used with RoboSep™ for automated immunomagnetic cell isolation.
Advantages
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components
  • EasySepSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit (Catalog #17884)
    • EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit with Filter Tips (Catalog #17884RF)
    • EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Centrifugation Tubes
Cell Type
Myeloid Cells
Species
Human
Sample Source
Whole Blood
Selection Method
Positive
Brand
EasySep
Area of Interest
Chimerism, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884RF
Lot #
All
Language
English

Educational Materials (10)

Brochure
EasySep™ Cell Separation Technology
Brochure
HLA Antibodies for Purity Assessment
Brochure
Cell Separation Products for HLA Analysis
Technical Bulletin
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
56:02
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Data and Publications

Data

Starting with human whole blood, the myeloid cell content of the isolated fraction is typically 94.5 ± 4.1% (gated on CD45; mean ± SD using the “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 66.6% and 98.5%, respectively.

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