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EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

From: 855 USD
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Immunomagnetic positive selection cell isolation kit
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Product Advantages


  • Fast and easy-to-use

  • Up to 99% purity

  • No columns required

What's Included

  • EasySepSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit (Catalog #17884)
    • EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit with Filter Tips (Catalog #17884RF)
    • EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

Isolate highly purified human CD33+ and CD66b+ myeloid cells, including myeloid progenitor cells, granulocytes, and monocytes, from fresh human whole blood or buffy coat samples by immunomagnetic positive selection, with the EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD33, CD66b, and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD33+ and CD66+ myeloid cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction for lineage-specific chimerism analysis.

This product has replaced the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA) for faster cell isolations.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™ to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Centrifugation Tubes
Cell Type
Myeloid Cells
Species
Human
Sample Source
Whole Blood
Selection Method
Positive
Brand
EasySep
Area of Interest
Chimerism, Immunology

Data Figures

Starting with human whole blood, the myeloid cell content of the isolated fraction is typically 94.5 ± 4.1% (gated on CD45; mean ± SD using the “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 66.6% and 98.5%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Catalog #
17884
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (11)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Prepare a Buffy Coat from Whole Blood
3:46
Video
How to Prepare a Buffy Coat from Whole Blood
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
The Nuts and Bolts of Lineage Chimerism Testing
33:41
Webinar
The Nuts and Bolts of Lineage Chimerism Testing
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
56:02
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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