EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

From: 656 USD
Catalog #
17873_C
Immunomagnetic positive selection cell isolation kit

Product Advantages


  • Fast and easy-to-use

  • Up to 99.9% purity

  • No columns required

What's Included

EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit (Catalog #17873)
    • EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
RoboSep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit (Catalog #17873RF)
    • EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Overview

The EasySep™ Human Whole Blood Lymphoid Positive Selection Kit is designed to isolate CD3+ and CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3, CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T lymphocytes and NKT cells while the CD19 antigen is expressed on B cells and follicular dendritic cells.

This product replaces the EasySep™ Human Whole Blood Lymphoid Positive Selection Kit (Catalog #18684) and EasySep™ HLA Whole Blood Lymphoid Positive Selection Kit (Catalog #18684HLA) for even faster cell isolations.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Lymphocytes, T Cells
Species
Human
Sample Source
Buffy Coat, Peripheral Blood, Whole Blood
Selection Method
Positive
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Starting with human whole blood, the lymphoid cell content (CD3+CD19+) of the isolated fraction is typically 99.5 ± 0.2% (gated on CD45; mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 16.2% and 98.5%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Safety Data Sheet 1
Catalog #
17873
Lot #
All
Language
English
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Safety Data Sheet 2
Catalog #
17873
Lot #
All
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English
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Safety Data Sheet 3
Catalog #
17873
Lot #
All
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English
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Safety Data Sheet 4
Catalog #
17873
Lot #
All
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English
Document Type
Safety Data Sheet 1
Catalog #
17873RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17873RF
Lot #
All
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English
Document Type
Safety Data Sheet 3
Catalog #
17873RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
17873RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Contact STEMCELL Technologies

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