ImmunoCult™ Immune Cell Culture
ImmunoCult™ Cell Culture
Activation, Expansion, Maintenance and Differentiation of Immune Cells
Ensure optimal activation, expansion, and differentiation of immune cell subsets using ImmunoCult™ products. These immune cell culture media, activators, and supplements allow you to culture various cell types, including T cells, B cells, NK cells, dendritic cells, and macrophages, under defined stimulatory conditions for consistent and reliable results.
Why Use ImmunoCult™?
- Activate, expand, or differentiate immune cells in culture conditions optimized to promote high yield and frequency.
- Minimize variation by using serum-free and animal-component free culture conditions.
- Consistently achieve high yields of immune cells with the desired phenotype and function.
- Mix and match media, activators, and supplements to suit your specific research needs.
ImmunoCult™ for Activation, Expansion, and Differentiation of T Cells
T cells play a vital role in regulating the immune response, and research on these cells is essential for understanding the complex responses to various pathogens and diseases, enabling development of new treatments and cures. Advance your fundamental T cell immunology and clinical T cell engineering research with products designed to ensure optimal culture conditions for human and mouse T cells. Culture cells under defined stimulatory conditions with specialized media, activators, and supplements to activate, edit, expand, or differentiate your cell population of interest.
GMP ImmunoCult™-XF
Products
Application:
Description:
Advantages:
- Confidently expand T cells for use in clinical applications by using medium produced under relevant GMPs
- Reduce variability by expanding cells in serum- and xeno-free culture conditions
- Achieve robust T cell expansion with high viability
- Obtain T cells able to produce cytokines upon restimulation
- Activate T cells bead-free by combining with ImmunoCult™ Human T Cell Activators
GMP ImmunoCult™ Human T Cell Activators
Products
Application:
Description:
Advantages:
- Generate robust activation and expansion of human T cells
- Scale seamlessly from research to clinical applications with a GMP-manufactured reagent designed for translational workflows
- Simplify protocols and reduce process complexity by eliminating magnetic beads or accessory reagents
- Preserve cell viability and functionality with gentle stimulation
ImmunoCult™-XF T Cell Expansion Medium
Application:
Description:
Advantages:
- Eliminate the need to supplement the medium with serum
- Achieve robust T cell expansion at levels comparable to those in serum-containing media
- Maintain CD4⁺/CD8⁺ cell proportions comparable to the start of culture following expansion
- Enable cytokine production in expanded T cells, including IFN-gamma and IL-4
ImmunoCult™ Human T Cell Activators
Products:
Application:
Description:
Advantages:
- Achieve robust activation and expansion of human T cells without the use of magnetic beads feeder cells, or antigen
- Maintain high viability of activated and expanded T cells with a gentle activation stimulus
- Rely on a highly stable, filter-sterilized soluble reagent for reproducible results
ImmunoCult™
Human T Cell Differentiation Supplements
Products:
Application:
Description:
Advantages:
- Induce human helper T cell differentiation with a formulation specifically designed for this purpose
- Optimize performance by pairing with ImmunoCult™-XF T Cell Expansion Medium
- Support significantly higher levels of memory T cells compared to competitor differentiation kits
- Simplify preparation with a 50X concentrate that can be added directly to the medium after thawing and mixing
ImmunoCult™ Mouse T Cell Activator Kit
Application:
Description:
Advantages:
- Activate mouse T cells with confidence using a single kit that allows for flexible design
- Achieve optimal activation without the use of magnetic beads, feeder cells, or antigen
- Maintain functional T cells with high viability by using a gentle activation stimulus
- Rely on a highly stable, filter-sterilized, soluble reagent that is compatible with common T cell growth media
ImmunoCult™
Mouse T Cell Differentiation Supplements
Products:
Application:
Description:
Advantages:
- Induce Th1, Th2, or Treg cells from naïve CD4⁺ T cells isolated from the spleen of a C57BL/6 mouse using an optimized formulation
- Simplify preparation with a 100X concentrate that can be added directly to the medium after thawing and mixing
The ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator is just excellent. When combined with RosetteSep™ Human CD4+ T Cell Enrichment Cocktail the resulting cultures are like Mt Fuji. That's how well that combination works. The data that we generated using this activator was the highlight of our publication. We really liked this reagent.
Kazuo Suzuki, St. Vincent's Centre for Applied Medical Research
Reagents for T Cell Therapy Research
The complex process of producing human T cells for cell therapy offers opportunities to optimize for maximum yield while retaining desired end phenotype and function. Explore additional optimized products for human T cell research, including tools for T cell isolation, genome editing, and cryopreservation.
ImmunoCult™ for NK Cell Expansion
The ability to culture and expand NK cells has become a useful tool for researching the use of NK cells for adoptive immunotherapy in cancer patients as well as understanding basic NK cell biology. Optimize your culture conditions and expand human NK cells with high yields in feeder- and serum-free culture conditions.
ImmunoCult™ NK Cell Expansion Kit
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Description:
Advantages:
- Expand NK cells robustly with high yield and frequency
- Improve control and consistency with feeder- and serum-free culture conditions
- Generate functional NK cells with cytotoxic potential
ImmunoCult™ for B Cell Expansion
The ability to culture and expand B cells in vitro has become a useful tool to study human immunity, B cell biology, and vaccine and therapeutic antibody development. Culture and expand mouse or human B cells in the absence of serum, feeder cells, or specialized culture plates.
ImmunoCult™ Human B Cell Expansion Supplement
Application:
Description:
Advantages:
- Achieve robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates
- Support consistent and reliable B cell culture with a serum- and xeno-free medium
ImmunoCult™ Human B Cell Expansion Kit
Application:
Description:
Advantages:
- Expand B cells in culture conditions optimized to promote high yield and frequency
- Minimize variation by using serum-free and animal-component free culture conditions
- Consistently achieve high yields of B cells
ImmunoCult™-XF B Cell Base Medium
Application:
Description:
Advantages:
- Achieve robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates
- Support consistent and reliable B cell culture with a serum- and xeno-free medium
ImmunoCult™ Mouse B Cell Expansion Kit
Application:
Description:
Advantages:
- Achieve robust in vitro expansion of mouse B cells without the use of serum, feeder cells, or specialized culture plates
- Simplify your workflow, with an optimized and easy-to-use culture system
- Obtain consistent yields of expanded mouse B cells
ImmunoCult™ for Dendritic Cell Generation
Dendritic cells (DCs) are potent antigen-presenting cells and key regulators of the immune response. These cells are of great interest for research in cancer immunotherapy, vaccines, and infectious diseases. Streamline your research on DCs with products designed for robust generation of mature DCs from isolated monocytes.
ImmunoCult™ Dendritic Cell Culture Kit
Application:
Description:
Advantages:
- Eliminate the need to supplement with serum by using animal component-free and serum-free medium
- Differentiate CD14+ monocytes into immature and subsequently mature DCs with an optimized formulation
- Achieve high yields of mature DCs with the desired phenotype
- Generate functional, mature DCs that produce the pro-inflammatory cytokine IL-12, and induce T cell proliferation
ImmunoCult™ Dendritic Cell Medium
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Application:
Description:
Advantages:
- Eliminate the need to supplement the medium with serum
- Support DC differentiation and maturation
- Use on its own or combine with differentiation and maturation supplements
ImmunoCult™ Differentiation Supplement
Application:
Description:
Advantages:
- Rely on a defined formulation that has been standardized and optimized
- Promote consistent differentiation of monocytes into immature dendritic cells
- Simplify your workflow with a ready-to-use differentiation supplement that is added directly to the medium
ImmunoCult™ Maturation Supplement
Application:
Description:
Advantages:
- Induce maturation of immature dendritic cells
- Streamline your workflow with a ready-to-use and optimized formulation
- Obtain high yields of mature DCs
- Generate mature DCs that produce cytokines and induce T cell proliferation
ImmunoCult™ for Macrophage Differentiation
Macrophages are cells of the innate immune system, able to respond to infections or injury through phagocytosis as well as through the immunomodulatory cytokines they produce. Macrophages such as the “classically activated” M1 macrophages and “alternatively activated” M2 macrophages are cell types of great interest due to their role in immune regulation, tissue repair, and tumor biology. Streamline your research on macrophages with culture medium designed for robust generation of M1 or M2 macrophages from isolated monocytes.
ImmunoCult™
Macrophage Medium
Application:
Description:
Advantages:
- Eliminate the need to supplement with serum
- Support robust macrophage differentiation
- Obtain high yields of macrophages with the desired phenotype and function
- Collect M1 or M2 macrophages in as little as 6 days
Antibody Finder Tool
Find the Right Antibody for Your Applications
Don't waste valuable research time finding the appropriate antibody for your cell analysis. Use our Antibody Finder Tool to select high-quality primary and secondary antibodies verified to work with ImmunoCult™ products in specific applications.
View Tool >Featured Resources
On-Demand Course on Human T Cell Expansion
Learn how to optimize your T cell culture and expansion protocol by incorporating ImmunoCult™ media and activators in this free self-paced course.
Additional Resources
