ImmunoCult™

Activation, Expansion, Maintenance and Differentiation of Immune Cells

Ensure optimal activation, expansion, and differentiation of immune cell subsets using ImmunoCult™ products. These immune cell culture media, activators, and supplements allow you to culture various cell types, including T cells, B cells, dendritic cells, and macrophages, under defined stimulatory conditions for consistent and reliable results.

Why Use ImmunoCult™?

  • Serum-free and animal-component-free medium.
  • Ready-to-use and optimized formulation for activation, expansion or differentiation of immune cells.
  • Results in a high yield of cells with the desired phenotype and function without the need to supplement with serum.
  • Media, activators and supplements can be used on their own or combined.

ImmunoCult™ for Activation, Expansion, and Differentiation of T Cells

Advance your basic T cell immunology and clinical T cell engineering research with products designed for:

  • Robust T cell activation and expansion without the use of magnetic beads, feeder cells, or antigens.
  • Consistent T cell expansion in serum- and xeno-free or animal component-free medium.
  • Differentiation of Th1, Th2, or Treg cells.

ImmunoCult™
Human T Cell Activators


Application:

Activation and Expansion of T Cells

Description:

Soluble antibody complexes that recognize and cross-link CD3 and co-stimulatory molecules on the surface of the T cell in the absence of magnetic beads or feeder cells. It can be used in combination with ImmunoCult™-XF T Cell Expansion Medium or any other media for culturing human T cells.

Advantages:

  • Robust activation and expansion of human T cells without the use of magnetic beads, feeder cells or antigen
  • Provides a gentle activation stimulus that maintains high viability of activated and expanded T cells
  • Highly stable, filter-sterilized soluble reagent

ImmunoCult™
Human T Cell Expansion Medium


ImmunoCult™ Human T Cell Expansion Medium

Application:

Expansion of T Cells

Description:

Serum-free and xeno-free medium optimized for the in vitro culture and expansion of human T cells isolated from peripheral blood. It can be used in combination with ImmunoCult™ T Cell Activators for bead-free activation of T cells.

Advantages:

  • No need to supplement the medium with serum
  • Supports robust T cell expansion at levels comparable to those in serum-containing media
  • Maintains similar proportions of CD4+/CD8+ cells to the start of culture after expansion
  • Expanded T cells are able to produce cytokines including IFN-gamma and IL-4

ImmunoCult™
Human T Cell Differentiation Supplements


ImmunoCult™ Human T Cell Differentiation Supplements

Application:

Differentiation of T Helper Cell Subsets

Description:

Differentiation supplements containing human cytokines and blocking antibodies formulated to promote the robust activation, expansion, and differentiation of peripheral blood-derived, naïve, CD4+ human T cells into Th1, Th2 or Treg cells.

Advantages:

  • Formulated for the induction of human T helper cell differentiation
  • Optimized for use with ImmunoCult™-XF T Cell Expansion Medium
  • Support significantly higher levels of memory T cells in comparison to competitor differentiation kits
  • Supplied as a 50X concentrate; after thawing and mixing, the tube contents can be added directly to medium

ImmunoCult™
Mouse T Cell Differentiation Supplements


ImmunoCult™ Mouse T Cell Differentiation Supplements

Application:

Differentiation of T Helper Cell Subsets

Description:

Differentiation supplements containing cytokines and blocking antibodies formulated to promote the differentiation of mouse naïve CD4+ T cells into Th1, Th2 or Treg cells.

Advantages:

  • Optimized for the induction of Th1, Th2 or Treg cells from naïve CD4+ T cells isolated from the spleen of a C57BL/6 mouse
  • Supplied as a 100X concentrate; after thawing and mixing, the tube contents can be added directly to medium
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Human T cell therapy workflow

Reagents for T Cell Therapy Research

The complex process of producing human T cells for cell therapy offers opportunities to optimize for maximum yield while retaining desired end phenotype and function. Explore additional optimized products for human T cell research, including tools for T cell isolation, genome editing, and cryopreservation.

Learn More >



ImmunoCult™ for B Cell Expansion

Expand human peripheral B cells without the use of serum, feeder cells, or specialized culture plates.

ImmunoCult™ Human B Cell Expansion Supplement


Application:

Expansion of B cells

Description:

Serum-free and animal component-free supplement formulated to promote robust in vitro expansion of human peripheral blood B cells in the absence of serum, feeder cells, or specialized culture plates. The supplement contains recombinant proteins and chemically defined components that support the activation and expansion of human B cells and their maturation to plasma cells.

Advantages:

  • Robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates
  • Serum-free and animal component-free supplement
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ImmunoCult™ for Dendritic Cell Generation

Streamline your research on dendritic cells (DCs) with products designed for robust generation of mature DCs from isolated monocytes.

ImmunoCult™ Dendritic Cell Medium

Application:

Culture, differentiation and maturation of dendritic cells (DCs)

Description:

Serum-free and animal-component free (ACF) medium optimized for the in vitro culture and differentiation of human monocytes into dendritic cells. It can be used in combination with ImmunoCult™ DC Differentiation and Maturation Supplements.

Advantages:

  • No need to supplement the medium with serum
  • Supports DC differentiation and maturation
  • Can be used on its own or combined with differentiation and maturation supplements

ImmunoCult™ Differentiation Supplement

ImmunoCult™ Dendritic Cell Differentiation Supplement

Application:

Differentiation of monocytes to immature DCs

Description:

Contains a combination of animal component-free (ACF) recombinant human cytokines formulated to support the differentiation of immature DCs from human monocytes.

Advantages:

  • Defined formulation that has been standardized and optimized
  • Promotes consistent differentiation of monocytes into immature dendritic cells
  • The differentiation supplement is ready-to-use and is simply added to the medium

ImmunoCult™ Maturation Supplement

ImmunoCult™ Dendritic Cell Maturation Supplement

Application:

Maturation of immature to mature DCs

Description:

Formulated to support the maturation of immature dendritic cells.

Advantages:

  • Induces maturation of immature dendritic cells
  • Ready-to-use and optimized formulation
  • Obtain high yields of mature DCs
  • Mature DCs produce cytokines and induce T cell proliferation
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ImmunoCult™ for Macrophage Differentiation

Streamline your research on macrophages with culture medium designed for robust generation of M1 or M2 macrophages from isolated monocytes.

ImmunoCult™
Macrophage Medium


Application:

Culture and Differentiation of Macrophages

Description:

Serum-free medium optimized for the in vitro culture and differentiation of human monocytes into macrophages. This medium can be combined with the appropriate cytokines and stimuli to differentiate M1 and M2a macrophages.

Advantages:

  • Serum-free medium without the need to supplement with serum
  • Supports robust macrophage differentiation
  • High yields of macrophages with the desired phenotype and function
  • Collect M1 or M2 macrophages in as little as 6 days
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