ImmunoCult™ Immune Cell Culture
ImmunoCult™ Cell Culture
Activation, Expansion, Maintenance and Differentiation of Immune Cells
Ensure optimal activation, expansion, and differentiation of immune cell subsets using ImmunoCult™ products. These immune cell culture media, activators, and supplements allow you to culture various cell types, including T cells, B cells, dendritic cells, and macrophages, under defined stimulatory conditions for consistent and reliable results.
Why Use ImmunoCult™?
- Activate, expand, or differentiate immune cells in culture conditions optimized to promote high yield and frequency.
- Minimize variation by using serum-free and animal-component free culture conditions.
- Consistently achieve high yields of immune cells with the desired phenotype and function.
- Mix and match media, activators, and supplements to suit your specific research needs.
ImmunoCult™ for Activation, Expansion, and Differentiation of T Cells
Advance your basic T cell immunology and clinical T cell engineering research with products designed for:
- Robust T cell activation and expansion without the use of magnetic beads, feeder cells, or antigens.
- Consistent T cell expansion in serum- and xeno-free or animal component-free medium.
- Differentiation of Th1, Th2, or Treg cells.
ImmunoCult™
Human T Cell Activators
Products:
Application:
Activation and Expansion of T Cells
Description:
Soluble antibody complexes that recognize and cross-link CD3 and co-stimulatory molecules on the surface of the T cell in the absence of magnetic beads or feeder cells. It can be used in combination with ImmunoCult™-XF T Cell Expansion Medium or any other media for culturing human T cells.
Advantages:
- Robust activation and expansion of human T cells without the use of magnetic beads, feeder cells or antigen
- Provides a gentle activation stimulus that maintains high viability of activated and expanded T cells
- Highly stable, filter-sterilized soluble reagent
ImmunoCult™
Human T Cell Expansion Medium
Application:
Expansion of T Cells
Description:
Serum-free and xeno-free medium optimized for the in vitro culture and expansion of human T cells isolated from peripheral blood. It can be used in combination with ImmunoCult™ T Cell Activators for bead-free activation of T cells.
Advantages:
- No need to supplement the medium with serum
- Supports robust T cell expansion at levels comparable to those in serum-containing media
- Maintains similar proportions of CD4+/CD8+ cells to the start of culture after expansion
- Expanded T cells are able to produce cytokines including IFN-gamma and IL-4
ImmunoCult™
Human T Cell Differentiation Supplements
Products:
Application:
Differentiation of T Helper Cell Subsets
Description:
Differentiation supplements containing human cytokines and blocking antibodies formulated to promote the robust activation, expansion, and differentiation of peripheral blood-derived, naïve, CD4+ human T cells into Th1, Th2 or Treg cells.
Advantages:
- Formulated for the induction of human T helper cell differentiation
- Optimized for use with ImmunoCult™-XF T Cell Expansion Medium
- Support significantly higher levels of memory T cells in comparison to competitor differentiation kits
- Supplied as a 50X concentrate; after thawing and mixing, the tube contents can be added directly to medium
ImmunoCult™
Mouse T Cell Differentiation Supplements
Products:
Application:
Differentiation of T Helper Cell Subsets
Description:
Differentiation supplements containing cytokines and blocking antibodies formulated to promote the differentiation of mouse naïve CD4+ T cells into Th1, Th2 or Treg cells.
Advantages:
- Optimized for the induction of Th1, Th2 or Treg cells from naïve CD4+ T cells isolated from the spleen of a C57BL/6 mouse
- Supplied as a 100X concentrate; after thawing and mixing, the tube contents can be added directly to medium
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The ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator is just excellent. When combined with RosetteSep™ Human CD4+ T Cell Enrichment Cocktail the resulting cultures are like Mt Fuji. That's how well that combination works. The data that we generated using this activator was the highlight of our publication. We really liked this reagent.
Kazuo Suzuki, St. Vincent's Centre for Applied Medical Research
Reagents for T Cell Therapy Research
The complex process of producing human T cells for cell therapy offers opportunities to optimize for maximum yield while retaining desired end phenotype and function. Explore additional optimized products for human T cell research, including tools for T cell isolation, genome editing, and cryopreservation.
ImmunoCult™ for B Cell Expansion
Expand human peripheral B cells without the use of serum, feeder cells, or specialized culture plates.
ImmunoCult™ Human B Cell Expansion Supplement
Application:
Expansion of B cells
Description:
Serum-free and animal component-free supplement formulated to promote robust in vitro expansion of human peripheral blood B cells in the absence of serum, feeder cells, or specialized culture plates. The supplement contains recombinant proteins and chemically defined components that support the activation and expansion of human B cells and their maturation to plasma cells.
Advantages:
- Robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates
- Serum-free and animal component-free supplement
ImmunoCult™ Human B Cell Expansion Kit
Application:
Expansion of B cells
Description:
ImmunoCult™ Human B Cell Expansion Kit comprises ImmunoCult™-XF B Cell Base Medium and ImmunoCult™-ACF Human B Cell Expansion Supplement, which together ensure consistent activation and expansion of human B cells and their maturation to plasma cells. B cells can be harvested and used directly in downstream applications after just one week or less in culture.
Advantages:
- Expand B cells in culture conditions optimized to promote high yield and frequency
- Minimize variation by using serum-free and animal-component free culture conditions
- Consistently achieve high yields of B cells
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ImmunoCult™ for Dendritic Cell Generation
Streamline your research on dendritic cells (DCs) with products designed for robust generation of mature DCs from isolated monocytes.
ImmunoCult™ Dendritic Cell Medium
Products:
Application:
Culture, differentiation and maturation of dendritic cells (DCs)
Description:
Serum-free and animal-component free (ACF) medium optimized for the in vitro culture and differentiation of human monocytes into dendritic cells. It can be used in combination with ImmunoCult™ DC Differentiation and Maturation Supplements.
Advantages:
- No need to supplement the medium with serum
- Supports DC differentiation and maturation
- Can be used on its own or combined with differentiation and maturation supplements
ImmunoCult™ Differentiation Supplement
Application:
Differentiation of monocytes to immature DCs
Description:
Contains a combination of animal component-free (ACF) recombinant human cytokines formulated to support the differentiation of immature DCs from human monocytes.
Advantages:
- Defined formulation that has been standardized and optimized
- Promotes consistent differentiation of monocytes into immature dendritic cells
- The differentiation supplement is ready-to-use and is simply added to the medium
ImmunoCult™ Maturation Supplement
Application:
Maturation of immature to mature DCs
Description:
Formulated to support the maturation of immature dendritic cells.
Advantages:
- Induces maturation of immature dendritic cells
- Ready-to-use and optimized formulation
- Obtain high yields of mature DCs
- Mature DCs produce cytokines and induce T cell proliferation
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ImmunoCult™ for Macrophage Differentiation
Streamline your research on macrophages with culture medium designed for robust generation of M1 or M2 macrophages from isolated monocytes.
ImmunoCult™
Macrophage Medium
Application:
Culture and Differentiation of Macrophages
Description:
Serum-free medium optimized for the in vitro culture and differentiation of human monocytes into macrophages. This medium can be combined with the appropriate cytokines and stimuli to differentiate M1 and M2a macrophages.
Advantages:
- Serum-free medium without the need to supplement with serum
- Supports robust macrophage differentiation
- High yields of macrophages with the desired phenotype and function
- Collect M1 or M2 macrophages in as little as 6 days
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Scientific Resources
Technical Bulletin
Optimization of Human T Cell Expansion Protocol: Effects of Early Cell Dilution
Brochure
Tools to Streamline your Dendritic Cell Research
Technical Bulletin
Genome Editing of Human Primary T Cells Using CRISPR-Cas9
Brochure
Tools to Streamline your Macrophage Research
