ImmunoCult™ Immune Cell Culture
ImmunoCult™ Cell Culture
Activation, Expansion, Maintenance and Differentiation of Immune Cells
Ensure optimal activation, expansion, and differentiation of immune cell subsets using ImmunoCult™ products. These immune cell culture media, activators, and supplements allow you to culture various cell types, including T cells, B cells, dendritic cells, and macrophages, under defined stimulatory conditions for consistent and reliable results.
Why Use ImmunoCult™?
- Activate, expand, or differentiate immune cells in culture conditions optimized to promote high yield and frequency.
- Minimize variation by using serum-free and animal-component free culture conditions.
- Consistently achieve high yields of immune cells with the desired phenotype and function.
- Mix and match media, activators, and supplements to suit your specific research needs.
ImmunoCult™ for Activation, Expansion, and Differentiation of T Cells
Advance your basic T cell immunology and clinical T cell engineering research with products designed for:
- Robust T cell activation and expansion without the use of magnetic beads, feeder cells, or antigens.
- Consistent T cell expansion in serum- and xeno-free or animal component-free medium.
- Differentiation of Th1, Th2, or Treg cells.
ImmunoCult™
Human T Cell Activators
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Advantages:
- Robust activation and expansion of human T cells without the use of magnetic beads, feeder cells or antigen
- Provides a gentle activation stimulus that maintains high viability of activated and expanded T cells
- Highly stable, filter-sterilized soluble reagent
ImmunoCult™
Human T Cell Expansion Medium
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Description:
Advantages:
- No need to supplement the medium with serum
- Supports robust T cell expansion at levels comparable to those in serum-containing media
- Maintains similar proportions of CD4+/CD8+ cells to the start of culture after expansion
- Expanded T cells are able to produce cytokines including IFN-gamma and IL-4
ImmunoCult™
Human T Cell Differentiation Supplements
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Advantages:
- Formulated for the induction of human T helper cell differentiation
- Optimized for use with ImmunoCult™-XF T Cell Expansion Medium
- Support significantly higher levels of memory T cells in comparison to competitor differentiation kits
- Supplied as a 50X concentrate; after thawing and mixing, the tube contents can be added directly to medium
ImmunoCult™
Mouse T Cell Differentiation Supplements
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Advantages:
- Optimized for the induction of Th1, Th2 or Treg cells from naïve CD4+ T cells isolated from the spleen of a C57BL/6 mouse
- Supplied as a 100X concentrate; after thawing and mixing, the tube contents can be added directly to medium
The ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator is just excellent. When combined with RosetteSep™ Human CD4+ T Cell Enrichment Cocktail the resulting cultures are like Mt Fuji. That's how well that combination works. The data that we generated using this activator was the highlight of our publication. We really liked this reagent.
Kazuo Suzuki, St. Vincent's Centre for Applied Medical Research
Reagents for T Cell Therapy Research
The complex process of producing human T cells for cell therapy offers opportunities to optimize for maximum yield while retaining desired end phenotype and function. Explore additional optimized products for human T cell research, including tools for T cell isolation, genome editing, and cryopreservation.
ImmunoCult™ for B Cell Expansion
Expand human peripheral B cells without the use of serum, feeder cells, or specialized culture plates.
ImmunoCult™ Human B Cell Expansion Supplement
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Description:
Advantages:
- Robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates
- Serum-free and animal component-free supplement
ImmunoCult™ Human B Cell Expansion Kit
Application:
Description:
Advantages:
- Expand B cells in culture conditions optimized to promote high yield and frequency
- Minimize variation by using serum-free and animal-component free culture conditions
- Consistently achieve high yields of B cells
ImmunoCult™ for Dendritic Cell Generation
Streamline your research on dendritic cells (DCs) with products designed for robust generation of mature DCs from isolated monocytes.
ImmunoCult™ Dendritic Cell Medium
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Advantages:
- No need to supplement the medium with serum
- Supports DC differentiation and maturation
- Can be used on its own or combined with differentiation and maturation supplements
ImmunoCult™ Differentiation Supplement
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Description:
Advantages:
- Defined formulation that has been standardized and optimized
- Promotes consistent differentiation of monocytes into immature dendritic cells
- The differentiation supplement is ready-to-use and is simply added to the medium
ImmunoCult™ Maturation Supplement
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Description:
Advantages:
- Induces maturation of immature dendritic cells
- Ready-to-use and optimized formulation
- Obtain high yields of mature DCs
- Mature DCs produce cytokines and induce T cell proliferation
ImmunoCult™ for Macrophage Differentiation
Streamline your research on macrophages with culture medium designed for robust generation of M1 or M2 macrophages from isolated monocytes.
ImmunoCult™
Macrophage Medium
Application:
Description:
Advantages:
- Serum-free medium without the need to supplement with serum
- Supports robust macrophage differentiation
- High yields of macrophages with the desired phenotype and function
- Collect M1 or M2 macrophages in as little as 6 days
Antibody Finder Tool
Find the Right Antibody for Your Applications
Don't waste valuable research time finding the appropriate antibody for your cell analysis. Use our Antibody Finder Tool to select high-quality primary and secondary antibodies verified to work with ImmunoCult™ products in specific applications.
View Tool >Scientific Resources
