ImmunoCult™
ImmunoCult™
Activation, Expansion, Maintenance and Differentiation of Immune Cells
Advance your research with ImmunoCult™ products designed to ensure optimal activation, expansion and differentiation of the immune cells of your interest. These specialized media, activators and supplements allow you to culture immune cells under defined stimulatory conditions for consistent and reliable results.
ImmunoCult™ for T Cells
Advance your basic T cell immunology and clinical T cell engineering research with ImmunoCult™ products designed for:
- Robust T cell activation and expansion without the use of magnetic beads, feeder cells or antigens.
- Consistent T cell expansion in serum- and xeno-free or animal component-free medium.
- Differentiation of Th1, Th2 or Treg cells.
ImmunoCult™ for Dendritic Cells and Macrophages
Streamline your research on dendritic cells (DCs) and macrophages with ImmunoCult™ products designed for:
- Generation of mature DCs and macrophages from isolated monocytes.
- Obtaining high yields of mature DCs or macrophages with the desired phenotype and function.
Why Use ImmunoCult™?
- Serum-free and animal-component-free medium.
- Ready-to-use and optimized formulation for activation, expansion or differentiation of immune cells.
- Results in a high yield of cells with the desired phenotype and function without the need to supplement with serum.
- Media, activators and supplements can be used on their own or combined.
ImmunoCult™ for T Cells
ImmunoCult™
Human T Cell Activators
Products:
Application:
Activation and Expansion of T Cells
Description:
Soluble antibody complexes that recognize and cross-link CD3 and co-stimulatory molecules on the surface of the T cell in the absence of magnetic beads or feeder cells. It can be used in combination with ImmunoCult™-XF T Cell Expansion Medium or any other media for culturing human T cells.
Advantages:
- Robust activation and expansion of human T cells without the use of magnetic beads, feeder cells or antigen
- Provides a gentle activation stimulus that maintains high viability of activated and expanded T cells
- Highly stable, filter-sterilized soluble reagent
ImmunoCult™
Human T Cell Expansion Medium
Application:
Expansion of T Cells
Description:
Serum-free and xeno-free medium optimized for the in vitro culture and expansion of human T cells isolated from peripheral blood. It can be used in combination with ImmunoCult™ T Cell Activators for bead-free activation of T cells.
Advantages:
- No need to supplement the medium with serum
- Supports robust T cell expansion at levels comparable to those in serum-containing media
- Maintains similar proportions of CD4+/CD8+ cells to the start of culture after expansion
- Expanded T cells are able to produce cytokines including IFN-gamma and IL-4
ImmunoCult™
Human T Cell Differentiation Supplements
Products:
Application:
Differentiation of T Helper Cell Subsets
Description:
Differentiation supplements containing human cytokines and blocking antibodies formulated to promote the robust activation, expansion, and differentiation of peripheral blood-derived, naïve, CD4+ human T cells into Th1, Th2 or Treg cells.
Advantages:
- Formulated for the induction of human T helper cell differentiation
- Optimized for use with ImmunoCult™-XF T Cell Expansion Medium
- Support significantly higher levels of memory T cells in comparison to competitor differentiation kits
- Supplied as a 50X concentrate; after thawing and mixing, the tube contents can be added directly to medium
ImmunoCult™
Mouse T Cell Differentiation Supplements
Products:
Application:
Differentiation of T Helper Cell Subsets
Description:
Differentiation supplements containing cytokines and blocking antibodies formulated to promote the differentiation of mouse naïve CD4+ T cells into Th1, Th2 or Treg cells.
Advantages:
- Optimized for the induction of Th1, Th2 or Treg cells from naïve CD4+ T cells isolated from the spleen of a C57BL/6 mouse
- Supplied as a 100X concentrate; after thawing and mixing, the tube contents can be added directly to medium
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New Partnership Enabling Development and Manufacturing of T Cell Immunotherapies
GE Healthcare has partnered with STEMCELL Technologies Inc. to provide T-Cell reagents for commercial-scale cell therapy production. Under the agreement, GE Healthcare will commercialize cGMP-grade versions of STEMCELL Technologies’ T-Cell reagents for the isolation, activation, and culture of T-cells in clinical applications.
ImmunoCult™ for Generation of Dendritic Cells
ImmunoCult™ Dendritic Cell Medium
Products:
Application:
Culture, differentiation and maturation of dendritic cells (DCs)
Description:
Serum-free and animal-component free (ACF) medium optimized for the in vitro culture and differentiation of human monocytes into dendritic cells. It can be used in combination with ImmunoCult™ DC Differentiation and Maturation Supplements.
Advantages:
- No need to supplement the medium with serum
- Supports DC differentiation and maturation
- Can be used on its own or combined with differentiation and maturation supplements
ImmunoCult™ Differentiation Supplement
Application:
Differentiation of monocytes to immature DCs
Description:
Contains a combination of animal component-free (ACF) recombinant human cytokines formulated to support the differentiation of immature DCs from human monocytes.
Advantages:
- Defined formulation that has been standardized and optimized
- Promotes consistent differentiation of monocytes into immature dendritic cells
- The differentiation supplement is ready-to-use and is simply added to the medium
ImmunoCult™ Maturation Supplement
Application:
Maturation of immature to mature DCs
Description:
Formulated to support the maturation of immature dendritic cells.
Advantages:
- Induces maturation of immature dendritic cells
- Ready-to-use and optimized formulation
- Obtain high yields of mature DCs
- Mature DCs produce cytokines and induce T cell proliferation
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ImmunoCult™ for Differentiation of Macrophages
ImmunoCult™
Macrophage Medium
Application:
Culture and Differentiation of Macrophages
Description:
Serum-free medium optimized for the in vitro culture and differentiation of human monocytes into macrophages. This medium can be combined with the appropriate cytokines and stimuli to differentiate M1 and M2a macrophages.
Advantages:
- Serum-free medium without the need to supplement with serum
- Supports robust macrophage differentiation
- High yields of macrophages with the desired phenotype and function
- Collect M1 or M2 macrophages in as little as 6 days
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