Anti-Mouse CD8a Antibody, Clone 53-6.7

Rat monoclonal IgG2a antibody against mouse, toad CD8a

Anti-Mouse CD8a Antibody, Clone 53-6.7

Rat monoclonal IgG2a antibody against mouse, toad CD8a

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Rat monoclonal IgG2a antibody against mouse, toad CD8a
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Overview

The 53-6.7 antibody reacts with murine CD8a, a 32 - 34 kDa type I transmembrane glycoprotein which is a subunit of CD8. CD8 is a disulfide-bonded dimer, found either as a heterodimer of CD8a (α) and CD8b (β) subunits (i.e., αβ) or a homodimer (αα). CD8 acts as a co-receptor to the T cell receptor (TCR) during T cell activation by binding MHC Class I molecules presented by an antigen-presenting cell. It functions to strengthen the association between the TCR and MHC I-antigen complex and to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. The CD8a chain binds to the alpha-3 domain of class I MHC molecules. CD8 is expressed in the αβ form by a majority of thymocytes and a subset of mature peripheral blood T cells (T cytotoxic cells), and in the ββ form by γδ T cells, a subset of intestinal intraepithelial lymphocytes and dendritic cells. The 53-6.7 antibody reportedly blocks antigen presentation via MHC class I and inhibits IL-2-dependent T cell responses.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD8+ T Cell Isolation Kit (Catalog #19853) and EasySep™ Mouse CD90.2 Positive Selection Kit II (Catalog #18951).
Subtype
Primary Antibodies
Target Antigen
CD8a
Alternative Names
Ly-2, Lyt2, T8
Reactive Species
Mouse, Toad
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
T Cells, T Cells, CD8+
Species
Mouse, Other
Application
Cell Isolation, CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Area of Interest
Immunology
Clone
53-6.7
Gene ID
12525
Isotype
IgG2a, kappa

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60023BT, 60023BT.1
Lot #
All
Language
English
Catalog #
60023AZ, 60023AZ.1
Lot #
All
Language
English
Catalog #
60023
Lot #
All
Language
English
Catalog #
60023FI, 60023FI.1
Lot #
All
Language
English
Catalog #
60023PS.1, 60023PS
Lot #
All
Language
English
Catalog #
60023AD.1, 60023AD
Lot #
All
Language
English
Catalog #
60023PE.1, 60023PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60023BT, 60023BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60023AZ, 60023AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60023
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60023FI, 60023FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60023PS.1, 60023PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60023AD.1, 60023AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60023PE.1, 60023PE
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.