EasySep™ Mouse CD90.2 Positive Selection Kit II

Immunomagnetic positive selection kit
Catalog #
18951_C
Immunomagnetic positive selection kit
From: 726 USD
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Required Products
  1. EasyEights™ EasySep™ Magnet
    EasyEights™ EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Mouse CD90.2 Positive Selection Kit II is designed to isolate CD90.2+ (Thy1.2+) cells from single-cell suspensions of splenocytes or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD90.2 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

This product replaces the EasySep™ Mouse CD90.2 Positive Selection Kit (Catalog #18751) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
Advantages
• Fast and easy-to-use
• Up to 98% purity
• No columns required
• Isolated cells are not fluorochrome-labeled
Components
  • EasySep™ Mouse CD90.2 Positive Selection Kit II (Catalog #18951)
    • EasySep™ Mouse CD90.2 Positive Selection II Component A, 0.5 mL
    • EasySep™ Mouse CD90.2 Positive Selection II Component B, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • RoboSep™ Empty Vial
  • RoboSep™ Mouse CD90.2 Positive Selection Kit II (Catalog #18951RF)
    • EasySep™ Mouse CD90.2 Positive Selection II Component A, 0.5 mL
    • EasySep™ Mouse CD90.2 Positive Selection II Component B, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • RoboSep™ Empty Vial
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Mouse
Sample Source
Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse CD90.2 Positive Selection Kit II
Catalog #
18951
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse CD90.2 Positive Selection Kit II
Catalog #
18951RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse CD90.2 Positive Selection Kit II
Catalog #
18951
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse CD90.2 Positive Selection Kit II
Catalog #
18951
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse CD90.2 Positive Selection Kit II
Catalog #
18951
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse CD90.2 Positive Selection Kit II
Catalog #
18951RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse CD90.2 Positive Selection Kit II
Catalog #
18951RF
Lot #
All
Language
English

Educational Materials (8)

Brochure
EasySep™ Cell Separation Technology
Brochure
Mouse Cell Isolation
Brochure
Tools For Your Immunology Research
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Human Immune Cytokines
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Typical EasySep™ CD902 Positive Selection Profile

Figure 1. Typical EasySep™ CD90.2 Positive Selection II Profile

Starting with mouse splenocytes, the CD90.2+ cell content of the isolated fraction is typically 95.8 ± 1.5% (mean ± SD using the purple EasySep™ Magnet).

Publications (1)

Frontiers in immunology 2019 Macrophage Coordination of the Interferon Lambda Immune Response. S. A. Read et al.

Abstract

Lambda interferons (IFN-$\lambda$s) are a major component of the innate immune defense to viruses, bacteria, and fungi. In human liver, IFN-$\lambda$ not only drives antiviral responses, but also promotes inflammation and fibrosis in viral and non-viral diseases. Here we demonstrate that macrophages are primary responders to IFN-$\lambda$, uniquely positioned to bridge the gap between IFN-$\lambda$ producing cells and lymphocyte populations that are not intrinsically responsive to IFN-$\lambda$. While CD14+ monocytes do not express the IFN-$\lambda$ receptor, IFNLR1, sensitivity is quickly gained upon differentiation to macrophages in vitro. IFN-$\lambda$ stimulates macrophage cytotoxicity and phagocytosis as well as the secretion of pro-inflammatory cytokines and interferon stimulated genes that mediate immune cell chemotaxis and effector functions. In particular, IFN-$\lambda$ induced CCR5 and CXCR3 chemokines, stimulating T and NK cell migration, as well as subsequent NK cell cytotoxicity. Using immunofluorescence and cell sorting techniques, we confirmed that human liver macrophages expressing CD14 and CD68 are highly responsive to IFN-$\lambda$ ex vivo. Together, these data highlight a novel role for macrophages in shaping IFN-$\lambda$ dependent immune responses both directly through pro-inflammatory activity and indirectly by recruiting and activating IFN-$\lambda$ unresponsive lymphocytes.
View All Publications

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