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Anti-Mouse CD4 Antibody, Clone RM4-5

Rat monoclonal IgG2a antibody against mouse CD4

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Anti-Mouse CD4 Antibody, Clone RM4-5

Rat monoclonal IgG2a antibody against mouse CD4

From: 68 USD
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Rat monoclonal IgG2a antibody against mouse CD4
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Overview

The RM4-5 antibody reacts with mouse CD4, an ~55 kDa single-chain type 1 transmembrane glycoprotein and member of the immunoglobulin (Ig) superfamily; CD4 contains four extracellular Ig-like domains. CD4 is expressed at relatively high levels by most thymocytes and a subpopulation of T cells (T helper/inducer cells), and at lower levels on dendritic cells. In the mouse, CD4 is not expressed by monocytes/macrophages. CD4 binds to a non-polymorphic region of MHC II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition by enhancing the avidity of the association between the TCR and MHC II-antigen complex. CD4 also functions to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. Binding of the RM4-5 antibody to CD4 inhibits ligand binding in vitro; binding of the RM4-5 antibody can be blocked by the clone GK1.5 antibody.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD4+ T Cell Isolation Kit (Catalog #19852) and EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit (Catalog #18782).
Subtype
Primary Antibodies
Target Antigen
CD4
Alternative Names
L3T4, T4
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
T Cells, T Cells, CD4+, T Cells, Regulatory
Species
Mouse
Application
Cell Isolation, CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
RM4-5
Gene ID
12504
Isotype
IgG2a, kappa

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, Alexa Fluor® 488 (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (Catalog #60076AD; solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE; solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ; solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, Biotin followed by streptavidin (SAV) APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT) followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, FITC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI; solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, PerCP-Cy5.5
Catalog #
60017PS, 60017PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, Biotin
Catalog #
60017BT.1, 60017BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, APC
Catalog #
60017AZ.1, 60017AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, Alexa Fluor® 488
Catalog #
60017AD, 60017AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, FITC
Catalog #
60017FI.1, 60017FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, PE
Catalog #
60017PE, 60017PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5
Catalog #
60017
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, PerCP-Cy5.5
Catalog #
60017PS, 60017PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, Biotin
Catalog #
60017BT.1, 60017BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, APC
Catalog #
60017AZ.1, 60017AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, Alexa Fluor® 488
Catalog #
60017AD, 60017AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, FITC
Catalog #
60017FI.1, 60017FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, PE
Catalog #
60017PE, 60017PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5
Catalog #
60017
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (4)

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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