Anti-Human CD19 Antibody, Clone HIB19

Mouse monoclonal IgG1 antibody against human, chimpanzee CD19
Catalog #
60005_C
Mouse monoclonal IgG1 antibody against human, chimpanzee CD19
From: 70 USD

Overview

The HIB19 antibody reacts with CD19, an ~95 kDa type 1 transmembrane glycoprotein expressed on the surface of B cells throughout all stages of development, from early pre-B cells to plasma cells. Expression is down-regulated but persists in terminally differentiated plasma cells. CD19 is also found on follicular dendritic cells. By associating with CD21 and CD81, CD19 functions as a co-receptor for the B cell receptor and is involved in B cell activation and differentiation. Activation of CD19 is accompanied by phosphorylation of the cytoplasmic domain, which promotes binding to kinases and the induction of intracellular signaling cascades. Mutations in CD19 can result in severe immunodeficiency syndromes.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human CD19 Positive Selection Kit (Catalog #18054), EasySep™ Human Whole Blood CD19 Positive Selection Kit (Catalog #18084), EasySep™ HLA Whole Blood B Cell Positive Selection Kit (Catalog #18184HLA); partial blocking may be observed, as well as EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood (Catalog #19954HLA) and EasySep™ HLA Total Lymphocyte Enrichment: Complete Processing Kit for Whole Blood (Catalog #19961HLA).
Subtype
Primary Antibodies
Target Antigen
CD19
Alternative Names
B4
Reactive Species
Chimpanzee, Human
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Mouse
Cell Type
B Cells
Species
Human, Non-Human Primate
Application
Cell Isolation, CyTOF, Flow Cytometry, Functional Assay, Immunohistochemistry
Area of Interest
Immunology
Clone
HIB19
Gene ID
930
Isotype
IgG1, kappa

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19
Catalog #
60005
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19, Alexa Fluor® 488
Catalog #
60005AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19, APC
Catalog #
60005AZ, 60005AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19, Biotin
Catalog #
60005BT, 60005BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19, FITC
Catalog #
60005FI, 60005FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19, PE
Catalog #
60005PE, 60005PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19, PerCP-Cy5.5
Catalog #
60005PS, 60005PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19
Catalog #
60005
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19, Alexa Fluor® 488
Catalog #
60005AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19, APC
Catalog #
60005AZ, 60005AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19, Biotin
Catalog #
60005BT, 60005BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19, FITC
Catalog #
60005FI, 60005FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19, PE
Catalog #
60005PE, 60005PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD19 Antibody, Clone HIB19, PerCP-Cy5.5
Catalog #
60005PS, 60005PS.1
Lot #
All
Language
English

Educational Materials(5)

Brochure
HLA Antibodies for Purity Assessment
Brochure
Tools For Your Immunology Research
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Reliable Antibodies for Your Research
Wallchart
Human Immune Cytokines

Data and Publications

Data

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, Alexa Fluor® 488 and anti-human CD45 APC.
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, Alexa Fluor® 488. Histograms show labeling of the PBMCs (Start) and isolated cells (Isolated). Labeling with a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).
(C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood and labeled with Anti-Human CD19 Antibody, Clone HIB19, APC. Histograms show labeling of HetaSep™-treated whole blood cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, Biotin followed by streptavidin (SAV) APC (filled histogram) or a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC (black line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC is shown (open histogram).
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC is shown (open histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ Human Whole Blood CD19 Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, PE and antihuman CD45 APC.
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, PE. Histograms show labeling of the PBMCs (Start) and isolated cells (Isolated). Labeling with a mouse IgG1, kappa PE isotype control antibody is shown in the bottom panel (open histogram).

Data for Unconjugated

Figure 6. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI; filled histogram) or a mouse IgG1, kappa isotype control antibody followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa PerCP-Cy5.5 isotype control antibody (solid line histogram).

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