RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail

Immunodensity negative selection cocktail
Catalog #
15263HLA_C
Immunodensity negative selection cocktail
Request Pricing
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Required Products
  1. Lymphoprep™|07851
    Lymphoprep™

    Density gradient medium for the isolation of mononuclear cells

Overview

The RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail is designed to enrich lymphocytes from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD16, CD36, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified lymphocytes are present as a highly enriched population at the interface between the plasma and the buoyant density medium. The desired cells are immediately ready for flow cytometry crossmatch assays or other downstream applictions.

This product carries the CE mark and is available as a Class I in vitro diagnostic device in the European Union and Australia.

Please Note: In light of the new EU Regulation 2017/746 (IVDR) of the European Parliament and of the Council on in vitro diagnostic medical devices, which is expected to become effective in 2022, STEMCELL has conducted a complete review of our RosetteSep™ CE-IVD products. We have determined that our current CE-marked IVD RosetteSep™ products will no longer carry the CE mark as “For In-Vitro Diagnostic Use” and will be labelled “For Research Use Only” labelling. These changes will take effect in December 2021. For more information please contact the Quality Assurance and Regulatory Department at qaschangenote@stemcell.com.
Advantages
• Fast and easy-to-use
• Requires no special equipment or training
• Isolated cells are untouched
Components
  • RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail (Catalog #15263HLA)
    • RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail, 5 x 2 mL
  • RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail (Catalog #15283HLA)
    • RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail, 20 x 2 mL
Subtype
Cell Isolation Kits
Cell Type
Lymphocytes
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Negative
Application
Cell Isolation, In Vitro Diagnostic
Brand
RosetteSep
Area of Interest
HLA, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet 1
Product Name
RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail
Catalog #
15263HLA
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail
Catalog #
15263HLA
Lot #
All
Language
Multi
Document Type
Product Information Sheet 1
Product Name
RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail
Catalog #
15283HLA
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail
Catalog #
15283HLA
Lot #
All
Language
MULTI
Document Type
Safety Data Sheet
Product Name
RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail
Catalog #
15263HLA
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail
Catalog #
15283HLA
Lot #
All
Language
English

Educational Materials (7)

Brochure
Cell Separation Products for HLA Analysis
Technical Bulletin
Cell Separation Solutions for the Flow Cytometric Crossmatch Assay
Wallchart
Human Immune Cytokines
Video
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
2:19
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
Video
Cell Isolation in One Spin Using SepMate™ Tubes and RosetteSep™ Cell Separation Reagents
1:02
Cell Isolation in One Spin Using SepMate™ Tubes and RosetteSep™ Cell Separation Reagents
Video
Isolate Highly Purified Cells for HLA Analysis with RosetteSep™ Immunodensity Cell Isolation
1:21
Isolate Highly Purified Cells for HLA Analysis with RosetteSep™ Immunodensity Cell Isolation
Webinar
Optimize the Flow Cytometry Crossmatch Assay: Save Time Without Compromising Sensitivity
1:01:28
Optimize the Flow Cytometry Crossmatch Assay: Save Time Without Compromising Sensitivity

Frequently Asked Question

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.
Read More

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

RosetteSep™ Procedure

Figure 1. RosetteSep™ Procedure

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.