ThawSTAR® CFT2 Automated Thawing System

Automated cell thawing system for consistent thawing performance
ThawSTAR® CFT2 Automated Thawing System

Automated cell thawing system for consistent thawing performance

1 Each
Catalog # 100-0650
2,600 USD
ThawSTAR® CFT2 Transporter

Portable solution for the handling and transport of frozen vials from liquid nitrogen or -80°C freezer

1 Each
Catalog # 100-0642
310 USD
ThawSTAR® CFT2 Confirmation Vials

For use before and after each thaw session to document instrument performance and audit trail

20 Vials
Catalog # 100-0643
170 USD
ThawSTAR® CFT2 IOPQ Kit

Installation, operational, and performance qualification documentation and accessories to facilitate functional testing of ThawSTAR® CFT2

1 Kit
Catalog # 100-0730
1,615 USD

Overview

Increase confidence in your cell thawing workflow by ensuring sample sterility and consistent thawing performance by using the ThawSTAR® CFT2 Automated Thawing System. With a standardized thawing process that replaces manual, water bath-based thawing, ThawSTAR® eliminates the risk of contamination and delivers controlled thawing profiles. Using this convenient and compact system, you can thaw your cells directly in the biosafety cabinet. Simply insert a frozen cryogenic vial and retrieve it once the device alerts you at the end of the thaw cycle, after approximately 2.5 minutes.The ThawSTAR® CFT2 Automated Thawing System can be leveraged early in the R&D phase and scaled into commercial manufacturing.

During vial transport, protect your cells from transient warming events to ensure maximum cell health by using the ThawSTAR® CFT2 Transporter. It provides a portable solution for the handling and transport of frozen vials from long-term storage in vapor phase liquid nitrogen or in a -80°C freezer to downstream processing with the ThawSTAR® CFT2 Automated Thawing System.

ThawSTAR® CFT2 Automated Thawing System is compatible with a variety of 1.8 - 2 mL vials including, Corning® Cryogenic Vials with Orange Caps (Catalog #100-0091 and #38053).
Advantages
⦁ Obtain reproducible thawing profiles using a standardized thawing method
⦁ Eliminate risk of contamination from water bath-based thawing techniques
⦁ Thaw cells directly in the biosafety cabinet
⦁ Portable solution for the handling and transport of frozen vials
⦁ Convenient and compact format
⦁ Document instrument performance and generate an audit trail with ThawSTAR® CFT2 confirmation vials
Components
  • ThawSTAR® CFT2 Automated Thawing System (Catalog #100-0650)
    • Thawstar® CFT2 Automated Thawing System, 1 unit (Catalog #100-0640)
    • ThawSTAR® CFT2 Transporter, 1 (Catalog #100-0641)
    • ThawSTAR® CFT2 Power Supply and Adaptors (Catalog #100-0644)
    • ThawSTAR® CFT2 Confirmation Vials, 2 Vials
Application
Cryopreservation
Brand
ThawSTAR
Area of Interest
Cord Blood Banking, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
ThawSTAR® CFT2 Automated Thawing System
Catalog #
100-0650
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
ThawSTAR® CFT2 Transporter
Catalog #
100-0642
Lot #
All
Language
English
Document Type
Manual
Product Name
ThawSTAR® CFT2 Automated Thawing System
Catalog #
100-0650
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ThawSTAR® CFT2 Automated Thawing System
Catalog #
100-0650
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ThawSTAR® CFT2 Confirmation Vials
Catalog #
100-0643
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ThawSTAR® CFT2 IOPQ Kit
Catalog #
100-0730
Lot #
All
Language
English

Educational Materials (6)

Brochure
T Cell Reagents for Your Cellular Therapy Research
Brochure
Cryopreservation Media for Stem Cell Research
Protocol
How to Cryopreserve Peripheral Blood Mononuclear Cells (PBMCs)
Protocol
How to Thaw Frozen Primary Cells
Webinar
Nature Research Round Table: Standards for Pluripotent Stem Cell Banking
13:02
Nature Research Round Table: Standards for Pluripotent Stem Cell Banking
Webinar
Improving Reproducibility of Your hPSC Research by Generating a High-Quality Cell Bank
21:53
Improving Reproducibility of Your hPSC Research by Generating a High-Quality Cell Bank

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Bar graphs comparing the number of live cells recovered and percentage viability for peripheral blood mononuclear cells (PBMCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 107 vs. 2.96 x 107 cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for monocytes thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 107 vs. 1.89 x 107 cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for human pluripotent stem cells (hPSCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 106 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 105 vs. 9.35 x 105 cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

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