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ThawSTAR® CFT2 Confirmation Vials

For use before and after each thaw session to document instrument performance and audit trail

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ThawSTAR® CFT2 Confirmation Vials

For use before and after each thaw session to document instrument performance and audit trail

From: 205 USD
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For use before and after each thaw session to document instrument performance and audit trail
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Overview

Document the performance of the ThawSTAR® CFT2 Automated Thawing System using ThawSTAR® CFT2 Confirmation Vials. Use the ThawSTAR® CFT2 Confirmation Vials under the same conditions as your biospecimens to be thawed, to evaluate and record unit thaw performance before and after each thaw session and create an audit trail. For your convenience, ThawSTAR® CFT2 Confirmation Vials can be purchased individually or as part of the ThawSTAR® CFT2 Automated Thawing System. The ThawSTAR® CFT2 Automated Thawing System comes complete with two ThawSTAR® CFT2 Confirmation Vials, the ThawSTAR® CFT2 Transporter, and ThawSTAR® CFT2 Power Supply and Adaptors for a water-free and standardized cell thawing process. For performance qualification documentation and accessories required to facilitate functional testing, use the ThawSTAR® CFT2 IOPQ Kit (available for separate purchase).

For more information on protocols for cell thawing using ThawSTAR®, please explore the Technical Manual.
Application
Cryopreservation
Brand
ThawSTAR
Area of Interest
Cord Blood Banking, Immunology

Data Figures

Bar graphs comparing the number of live cells recovered and percentage viability for peripheral blood mononuclear cells (PBMCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 107 vs. 2.96 x 107 cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for monocytes thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 107 vs. 1.89 x 107 cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for human pluripotent stem cells (hPSCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 106 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 105 vs. 9.35 x 105 cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Technical Manual
Product Name
ThawSTAR® CFT2 Confirmation Vials
Catalog #
100-0643
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ThawSTAR® CFT2 Confirmation Vials
Catalog #
100-0643
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (6)

Cryopreservation Media for Stem Cell Research
Brochure
Cryopreservation Media for Stem Cell Research
How to Thaw Frozen Primary Cells
Protocol
How to Thaw Frozen Primary Cells
How to Cryopreserve Peripheral Blood Mononuclear Cells (PBMCs)
Protocol
How to Cryopreserve Peripheral Blood Mononuclear Cells (PBMCs)
How to Quickly Thaw Frozen Cells with the ThawStar® CFT2 Automated Thawing System
1:49
Video
How to Quickly Thaw Frozen Cells with the ThawStar® CFT2 Automated Thawing System
Nature Research Round Table: Standards for Pluripotent Stem Cell Banking
13:02
Webinar
Nature Research Round Table: Standards for Pluripotent Stem Cell Banking
Improving Reproducibility of Your hPSC Research by Generating a High-Quality Cell Bank
21:53
Webinar
Improving Reproducibility of Your hPSC Research by Generating a High-Quality Cell Bank

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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