Human primary cells
are cells isolated directly from tissues, including peripheral blood, cord blood, and bone marrow. These cells are increasingly recognized for their importance in the study of biological processes, disease progression, and drug development, and for applications including in vitro cell-based assays or the creation of xenograft or humanized mouse models.
Fresh primary cells are often cryopreserved (i.e. frozen) for long-term storage in liquid nitrogen if they are not required immediately. Researchers with limited resources for processing fresh samples may also purchase frozen, ready-to-use primary cells, including mononuclear cells (MNCs), purified immune cells, or hematopoietic stem cells. When thawing frozen cells, proper technique and handling ensures optimal viability, recovery, and functionality of the cells for downstream applications.
This protocol describes how to thaw frozen primary cells. As thawing protocols for specific cell types may vary, always refer to the recommended protocol received with your cells.