Iscove's Modified Dulbecco's Medium

Iscove's Modified Dulbecco's Medium (IMDM) with 25 mM HEPES

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Iscove's Modified Dulbecco's Medium

Iscove's Modified Dulbecco's Medium (IMDM) with 25 mM HEPES

500 mL
Catalog #36150
58 USD

Overview

Iscove's Modified Dulbecco's Medium (IMDM) with 25 mM HEPES is recommended for a wide variety of cell culture applications. Selection of suitable nutrient medium is dependent on the type of cell, conditions of culture, and degree of chemical definition required for the cell culture application.
Subtype:
Basal Media
Cell Type:
Other
Species:
Human; Mouse; Rat; Non-Human Primate; Other
Application:
Cell Culture

Scientific Resources

Product Documentation

Educational Materials

(1)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Publications

(1)
Journal of immunology (Baltimore, Md. : 1950) 2011 JAN

SHIP represses Th2 skewing by inhibiting IL-4 production from basophils.

E. Kuroda et al.

Abstract

We report that SHIP(-/-) mice, compared to SHIP(+/+) mice, are Th2 skewed with elevated serum IgE and twice as many splenic CD4(+) Th2 cells that, when stimulated with anti-CD3, produce more IL-4 and less IFN-$\gamma$. Exploring the reason for this Th2 skewing, we found that freshly isolated SHIP(-/-) splenic and bone marrow basophils are present in elevated numbers and secrete far more IL-4 in response to IL-3 or to Fc$\epsilon$RI stimulation than do WT basophils. These SHIP(-/-) basophils markedly skew wild-type macrophage colony stimulating factor-derived macrophages toward an M2 phenotype, stimulate OT-II CD4(+) Th cells to differentiate into Th2 cells, and trigger SHIP(+/+) B cells to become IgE-producing cells. All these effects are completely abrogated with neutralizing anti-IL-4 Ab. Exploring the cell signaling pathways responsible for hyperproduction of IL-4 by SHIP(-/-) basophils, we found that IL-3-induced activation of the PI3K pathway is significantly enhanced and that PI3K inhibitors, especially a p110$\alpha$ inhibitor, dramatically suppresses IL-4 production from these cells. In vivo studies, in which basophils were depleted from mast cell-deficient SHIP(+/+) and SHIP(-/-) mice, confirmed the central role that basophils play in the Th2 skewing of naive SHIP-deficient mice. Taken together, these studies demonstrate that SHIP is a potent negative regulator of IL-4 production from basophils and thus may be a novel therapeutic target for Th1- and Th2-related diseases.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.