MesenCult™-ACF Freezing Medium

Animal component-free MSC cryopreservation medium
MesenCult™-ACF Freezing Medium

Animal component-free MSC cryopreservation medium

50 mL
Catalog # 05490
196 USD

Overview

MesenCult™-ACF Freezing Medium is a defined, serum-free, and animal component-free medium for the cryopreservation of mesenchymal stem cells (MSCs). This complete and ready-to-use medium is recommended for human MSCs previously cultured in MesenCult™-ACF Plus Medium (Catalog #05445) or MesenCult™ Medium (MesenCult™ Proliferation Kit; Catalog #05411). Frozen human MSCs should be stored at -135°C (liquid nitrogen) or colder.
Advantages
• Serum-free and animal component-free
• Reproducibly high recovery rates
• Optimized for MSCs previously cultured in MesenCult™-ACF Plus Medium or MesenCult™ Medium
• Preserves human MSC multipotency and expansion capacities
• Convenient, ready-to-use format
Contains
Dimethyl sulfoxide (DMSO)
Cell Type
Mesenchymal Stem and Progenitor Cells
Species
Human
Application
Cryopreservation
Brand
MesenCult
Area of Interest
Stem Cell Biology
Formulation
Animal Component-Free, Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
MesenCult™-ACF Freezing Medium
Catalog #
05490
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
MesenCult™-ACF Freezing Medium
Catalog #
05490
Lot #
All
Language
English

Educational Materials(6)

Brochure
Cryopreservation Media for Stem Cell Research
Brochure
Products for Your Mesenchymal Stromal Cell Research
Wallchart
The Identity and Properties of Mesenchymal Stem Cells
Video
1:51
MesenCult™ for Mesenchymal Stromal Cell Derivation, Culture & Differentiation
Video
13:45
Isolation of Umbilical Cord Mesenchymal Stromal Cells Using Explant Method
Webinar
58:00
The Secret In Vivo Life of "Mesenchymal Stem Cells"

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Human MSCs previously cultured in animal component-free medium and cryopreserved in MesenCult™-ACF Freezing Medium show reproducibly high recovery of viable cells after thawing. Recovery is higher than observed with a competitor ACF freezing medium (n = 6 independent experiments, P = 0.0035). Viable cell recovery is defined as the percentage of input cells that are recovered and maintain post-thaw viability.

Human MSCs cryopreserved with MesenCult™-ACF Freezing Medium (A, 3 days after thawing) maintain multi-lineage differentiation potential, as demonstrated by differentiation to the adipogenic (B, Oil Red O staining) and osteogenic (C, Alizarin Red staining) lineages.

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