MesenCult™-ACF Freezing Medium
Animal component-free MSC cryopreservation medium
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MesenCult™-ACF Freezing Medium -
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Overview
MesenCult™-ACF Freezing Medium is a defined, serum-free, and animal component-free medium for the cryopreservation of mesenchymal stem cells (MSCs). This complete and ready-to-use medium is recommended for human MSCs previously cultured in MesenCult™-ACF Plus Medium (Catalog #05445) or MesenCult™ Medium (MesenCult™ Proliferation Kit; Catalog #05411). Frozen human MSCs should be stored at -135°C (liquid nitrogen) or colder.
Advantages:
• Serum-free and animal component-free
• Reproducibly high recovery rates
• Optimized for MSCs previously cultured in MesenCult™-ACF Plus Medium or MesenCult™ Medium
• Preserves human MSC multipotency and expansion capacities
• Convenient, ready-to-use format
• Reproducibly high recovery rates
• Optimized for MSCs previously cultured in MesenCult™-ACF Plus Medium or MesenCult™ Medium
• Preserves human MSC multipotency and expansion capacities
• Convenient, ready-to-use format
Contains:
Dimethyl sulfoxide (DMSO)
Cell Type:
Mesenchymal Stem and Progenitor Cells
Species:
Human
Brand:
MesenCult
Area of Interest:
Stem Cell Biology
Formulation:
Animal Component-Free; Serum-Free
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MesenCult™ Adipogenic Differentiation Kit (Human)
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Animal Component-Free Cell Dissociation Kit
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MesenCult™-ACF Chondrogenic Differentiation Kit
Animal component-free medium for the differentiation of MSCs into chondrocytes
Scientific Resources
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Data
Human MSCs previously cultured in animal component-free medium and cryopreserved in MesenCult™-ACF Freezing Medium show reproducibly high recovery of viable cells after thawing. Recovery is higher than observed with a competitor ACF freezing medium (n = 6 independent experiments, P = 0.0035). Viable cell recovery is defined as the percentage of input cells that are recovered and maintain post-thaw viability.
Human MSCs cryopreserved with MesenCult™-ACF Freezing Medium (A, 3 days after thawing) maintain multi-lineage differentiation potential, as demonstrated by differentiation to the adipogenic (B, Oil Red O staining) and osteogenic (C, Alizarin Red staining) lineages.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.