MesenCult™-ACF Freezing Medium

Animal component-free MSC cryopreservation medium

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MesenCult™-ACF Freezing Medium

Animal component-free MSC cryopreservation medium

50 mL
Catalog #05490
187 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

MesenCult™-ACF Freezing Medium is a defined, serum-free, and animal component-free medium for the cryopreservation of mesenchymal stem cells (MSCs). This complete and ready-to-use medium is recommended for human MSCs previously cultured in MesenCult™-ACF Plus Medium (Catalog #05445) or MesenCult™ Medium (MesenCult™ Proliferation Kit; Catalog #05411). Frozen human MSCs should be stored at -135°C (liquid nitrogen) or colder.
Advantages:
• Serum-free and animal component-free
• Reproducibly high recovery rates
• Optimized for MSCs previously cultured in MesenCult™-ACF Plus Medium or MesenCult™ Medium
• Preserves human MSC multipotency and expansion capacities
• Convenient, ready-to-use format
Contains:
Dimethyl sulfoxide (DMSO)
Cell Type:
Mesenchymal Stem and Progenitor Cells
Species:
Human
Brand:
MesenCult
Area of Interest:
Stem Cell Biology
Formulation:
Animal Component-Free; Serum-Free

Scientific Resources

Product Documentation

Educational Materials

(6)
Brochure
Cryopreservation Media for Stem Cell Research
Brochure
Products for Your Mesenchymal Stromal Cell Research
Wallchart
The Identity and Properties of Mesenchymal Stem Cells
Wallchart
Roles for MSCs as Medicinal Signaling Cells
Video
1:51
MesenCult™ for Mesenchymal Stromal Cell Derivation, Culture & Differentiation
Webinar
58:00
The Secret In Vivo Life of "Mesenchymal Stem Cells"

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
hPSC-Derived Mesoderm Cell Research
Mesoderm Induction
Downstream Differentiation
Mesenchymal Stem and Progenitor Cell Research
Cell Sourcing and Isolation
Culture and Cryopreservation
Differentiation
Characterization
Product Portfolios
Cord Blood Banking
T Cell Engineering
Human Pluripotent Stem Cell Research
Hematopoietic Stem and Progenitor Cell Research
HLA Analysis
T Cell Research
B Cell Research
hPSC-Derived Neural Cell Research
Airway Epithelial Cell Research
Brain Tumor Stem Cell Research
CHO and Other Cell Line Development
Dendritic Cell Research
Granulocyte Research
hPSC-Derived Endoderm Cell Research
hPSC-Derived Mesoderm Cell Research
Hybridoma Production
Intestinal Epithelial Cell Research
Total Lymphocyte Research
Mammary Epithelial Cell Research
Mesenchymal Stem and Progenitor Cell Research
Monocyte Research
Mouse Pluripotent Stem Cell Research
Myeloid Cell Research
Neural Stem and Progenitor Cell Research
Neuronal Research
Natural Killer Cell Research
Prostate Epithelial Cell Research
Chimerism Analysis
Innate Lymphoid Cell Research
Myogenic Stem and Progenitor Cell Research
Organoids
T Cell Therapy Research
Mesenchymal Stromal Cell Therapy Research
Hematopoietic Cell Therapy Research
PSC Quality Control
Genome Editing
Cardiac and Skeletal Muscle Research
T Cell Therapy Research
Mesenchymal Stromal Cell Therapy Research
Mesenchymal Stromal Cell Therapy Research
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Data and Publications

Data

Human MSCs previously cultured in animal component-free medium and cryopreserved in MesenCult™-ACF Freezing Medium show reproducibly high recovery of viable cells after thawing. Recovery is higher than observed with a competitor ACF freezing medium (n = 6 independent experiments, P = 0.0035). Viable cell recovery is defined as the percentage of input cells that are recovered and maintain post-thaw viability.

Human MSCs cryopreserved with MesenCult™-ACF Freezing Medium (A, 3 days after thawing) maintain multi-lineage differentiation potential, as demonstrated by differentiation to the adipogenic (B, Oil Red O staining) and osteogenic (C, Alizarin Red staining) lineages.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.