Human Recombinant M-CSF
Overview
M-CSF exerts its biological effects by signaling through a receptor tyrosine kinase (CSF-1R or M-CSF-R) encoded by the c-fms proto-oncogene (Hamilton). CSF-1R shares similar structural features with other growth factor receptors, including the stem cell factor (SCF) receptor, platelet-derived growth factor receptor (PDGF-R), and Flt3/Flk-2 receptor tyrosine kinase. Stimulation of the CSF-1R upon binding to M-CSF activates MAPK, PI3K, and PLCγ signaling pathways (Chockalingam & Ghosh). Human and mouse M-CSF sequences are highly conserved both at nucleotide and amino acid levels (80% homology; DeLamarter et al.).
Scientific Resources
Product Documentation
Document Type | Product Name | Catalog # | Lot # | Language |
---|---|---|---|---|
Document Type
Product Information Sheet
|
Product Name
Human Recombinant M-CSF
|
Catalog #
78057, 78057.1, 78057.2 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet
|
Product Name
Human Recombinant M-CSF
|
Catalog #
78057, 78057.1, 78057.2 |
Lot #
All |
Language
English |
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

(A) The biological activity of Human Recombinant M-CSF was tested by its ability to promote the proliferation of NFS-60 cells. Cell
proliferation was measured after 44 hours of culture using a fluorometric assay method. The EC50 is defined as the effective
concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.9 - 1.4 ng/mL.
(B) 1 μg of Human Recombinant M-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized
by Coomassie Blue staining. Human Recombinant M-CSF is a homodimer of 18.5 kDa subunits with a predicted total molecular mass of
37.1 kDa.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.