Human Recombinant IL-1 beta

Interleukin 1 beta

Human Recombinant IL-1 beta

Interleukin 1 beta

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Interleukin 1 beta
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Overview

Interleukin 1 beta (IL-1β) is synthesized as an inactive precursor protein or pro-IL-1β. This precursor is cleaved intracellularly by caspase 1 (IL-1β convertase) to form the active form of the protein that is later secreted (Allan et al.). IL-1β binds to IL-1 receptor and activates intracellular signaling via the MAPK or NF-κB pathway. IL-1β is released by monocytes, tissue macrophages, and dendritic cells in response to infection or injury and induces expression of acute-phase proteins. It also promotes the infiltration of inflammatory and immunocompetent cells from the circulation into the extravascular space and affected tissues, by stimulating the expression of adhesion molecules on endothelial cells. IL-1β also affects other immune cells; for example, it co-stimulates T cell functions together with an antigen or mitogen. It also stimulates Th17 differentiation and B cell proliferation in an IL-6-dependent manner.
Subtype
Cytokines
Cell Type
B Cells, Hematopoietic Stem and Progenitor Cells, T Cells, T Cells, CD4+, T Cells, CD8+
Species
Human
Area of Interest
Immunology, Stem Cell Biology
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant IL-1 beta was tested by its ability to promote the proliferation of D10S cells. Cell proliferation was measured after 66 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.7 - 1 pg/mL.
(B) 1 μg of Human Recombinant IL-1 beta was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-1 beta has a predicted molecular mass of 17.5 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78034.3, 78034, 78034.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78034.3, 78034, 78034.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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