EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit
18-Minute cell isolation kit using immunomagnetic negative selection
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EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit -
Label for EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit is designed to isolate CD11b+Gr1+ myeloid-derived suppressor cells (MDSCs) from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-MDSCs and streptavidin-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, cell sorting, and cell culture.
Advantages:
• Fast, easy-to-use and column-free
• Up to 96% purity
• Untouched, viable cells
• Up to 96% purity
• Untouched, viable cells
Components:
- EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit (Catalog #19867)
- EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Cocktail, 0.5 mL
- EasySep™ Streptavidin RapidSpheres™ 50001, 1.0 mL
- EasySep™ Mouse FcR Blocker, 2 x 0.2 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype:
Cell Isolation Kits
Cell Type:
Granulocytes and Subsets; Monocytes; Myeloid Cells
Species:
Mouse
Sample Source:
Bone Marrow; Other; Whole Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology
Related Products
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Magnet for column-free immunomagnetic separation -
For detection and measurement of mouse interferon-gamma -
EasySep™ Mouse CD4+ T Cell Isolation Kit
15-Minute cell isolation kit using immunomagnetic negative selection -
EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II
Immunomagnetic negative selection followed by positive selection kit
Labeling Antibodies
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Anti-Mouse CD45 Antibody, Clone 30-F11
Rat monoclonal IgG2b antibody against mouse CD45 -
Anti-Mouse CD11b Antibody, Clone M1/70
Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b
Scientific Resources
Product Documentation
Document Type
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Catalog #
Lot #
Language
Educational Materials
(7)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. Typical EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit Profile from 4T1 Tumor-Bearing BALB/c Mouse Splenocytes
Starting with 4T1 tumor-bearing BALB/c mouse splenocytes, the MDSC content (CD11b+Gr1+) of the isolated fraction is typically 94.3 ± 2.1% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 20.7% and 94.7%, respectively.
Figure 2. Typical EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit Profile from Naïve C57BL/6 Mouse Splenocytes
Starting with naïve C57BL/6 mouse splenocytes, the CD11b+Gr1+ cell content of the isolated fraction is typically 86 ± 4.6% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 3.0% and 86.8%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.