EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit

18-Minute cell isolation kit using immunomagnetic negative selection

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit

18-Minute cell isolation kit using immunomagnetic negative selection

EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit
1 x 10^9 cells
778 USD
Catalog # 19867

Immunomagnetic isolation of untouched myeloid-derived suppressor cells (CD11b+Gr1+).

Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 96% purity

  • Untouched, viable cells

What's Included

  • EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit (Catalog #19867)
    • EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Cocktail, 0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1.0 mL
    • EasySep™ Mouse FcR Blocker, 2 x 0.2 mL
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Overview

The EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit is designed to isolate CD11b+Gr1+ myeloid-derived suppressor cells (MDSCs) from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-MDSCs and streptavidin-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, cell sorting, and cell culture.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets, Monocytes, Myeloid Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Figure 1. Typical EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit Profile from 4T1 Tumor-Bearing BALB/c Mouse Splenocytes

Starting with 4T1 tumor-bearing BALB/c mouse splenocytes, the MDSC content (CD11b+Gr1+) of the isolated fraction is typically 94.3 ± 2.1% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 20.7% and 94.7%, respectively.

Figure 2. Typical EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit Profile from Naïve C57BL/6 Mouse Splenocytes

Starting with naïve C57BL/6 mouse splenocytes, the CD11b+Gr1+ cell content of the isolated fraction is typically 86 ± 4.6% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 3.0% and 86.8%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
19867
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
19867
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
19867
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
19867
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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