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Anti-Mouse CD45 Antibody, Clone 30-F11

Rat monoclonal IgG2b antibody against mouse CD45

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Anti-Mouse CD45 Antibody, Clone 30-F11

Rat monoclonal IgG2b antibody against mouse CD45

From: 80 USD
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Rat monoclonal IgG2b antibody against mouse CD45
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Overview

The 30-F11 antibody reacts with an extracellular epitope found on all isoforms and both alloantigens (CD45.1 and CD45.2) of mouse CD45, a type I transmembrane glycoprotein expressed on the surface of most hematopoietic cells except mature erythrocytes, platelets and plasma cells; expression of CD45 is lost during differentiation of these cell types. CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including cell activation, growth, differentiation and oncogenic transformation. The large cytoplasmic portion of CD45 contains two tyrosine phosphatase domains, one which is enzymatically active, that are involved in modulating the function of intracellular substrates such as the Src kinases Lck and Fyn. Several isoforms of CD45 have been identified, the expression of which differs according to cell type and functional status. Alternative splicing of three exons (4, 5, 6) encoding the extracellular RA, RB and RC polypeptide sequences gives rise to up to 8 isoforms with molecular masses in the range of 180 - 240 kDa.
Subtype
Primary Antibodies
Target Antigen
CD45
Alternative Names
LCA, Leukocyte common antigen, Ly-5, Protein tyrosine phosphatase receptor type C, PTPRC, T200
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Lymphocytes, Myeloid Cells
Species
Mouse
Application
CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
30-F11
Gene ID
19264
Isotype
IgG2b, kappa

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488 (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (Catalog #60077AD) (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, PE (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (Catalog #60077PE) (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa PerCP-Cy5.5 isotype control antibody (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin
Catalog #
60030BT, 60030BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, PE
Catalog #
60030PE, 60030PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, PerCP-Cy5.5
Catalog #
60030PS.1, 60030PS
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, FITC
Catalog #
60030FI, 60030FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11
Catalog #
60030.1, 60030
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488
Catalog #
60030AD, 60030AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, APC
Catalog #
60030AZ.1, 60030AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin
Catalog #
60030BT, 60030BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, PE
Catalog #
60030PE, 60030PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, PerCP-Cy5.5
Catalog #
60030PS.1, 60030PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, FITC
Catalog #
60030FI, 60030FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11
Catalog #
60030.1, 60030
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488
Catalog #
60030AD, 60030AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, APC
Catalog #
60030AZ.1, 60030AZ
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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